Mammalian Protein Expression
Truly Functional Protein
95% Purity
Fast turnaround
Transient Expression
1-10 mg from CHO or 293 Cells
Start from $3950


Baculovirus Protein Expression
Functional Protein
95% Purity
Fast turnaround
1-10 mg from insect Sf9 cells
Start from $3950


High Titer Lentivirus
ORF, shRNA & Reporters
>10^9 titer
* Custom shRNA & cDNA Constructs
* Scramble shRNA
* Cre & FLP Recombinases
* Luciferases, Fluorescent Proteins
* Lentiviral Packaging Service
* Stable Cell Lines
* Induced Pluripotent Stem Cells


High Titer Adenovirus & AAV
ORF, shRNA & Reporters
>10^10 titer
* Custom shRNA & cDNA Constructs
* Scramble shRNA
* Cre & FLP Recombinases
* Luciferases, Fluorescent Proteins
* Transient Protein Expression
* Stable Cell Lines
* Induced Pluripotent Stem Cells

Excellgen

A Microsurgical Assistant System

Taylor, Russell H, Professor And Director
Computer Sciencejohns Hopkins University

Grant 1R01EB007969-01A1 from National Institute Of Biomedical Imaging And Bioengineering IRG: ZRG1

Abstract: This Bioengineering Research Partnership (BRP) will focus the efforts of highly qualified engineers, scientists and micro-surgeons from Johns Hopkins University and Carnegie-Mellon University to develop technology and systems addressing fundamental limitations in current microsurgical practice, using vitreoretinal surgery as our focus. Vitreoretinal surgery is the most technically demanding ophthalmologic discipline and addresses prevalent sight-threatening conditions in areas of growing need. With the aging of our population, the prevalence of sight-threatening conditions will continue to escalate. Retinal surgery is currently performed under an operating microscope with free-hand instrumentation. Limitations include limited visual resolution, and physiological hand tremor. The surgeon also struggles with a lack of tactile feedback, proximity sensing, and real-time sensing of physiological parameters of the retina. Surgical technique and efficiency would be enhanced by the integration of preoperative images with the intraoperative view. Poor ergonomics for the surgeon in current practice result in surgeon fatigue and potential disability. All of these factors contribute to extended operating times, attendant light toxicity, and higher than needed complication rates. At the center of our planned approach is a "surgical workstation" system interfaced to a stereo visualization subsystem and a family of novel sensors, instruments, and robotic devices. The capabilities of these components individually address important limitations of current practice; together they provide a modular, synergistic, and extendable system that enables computer-interfaced technology and information processing to work in partnership with surgeons to improve clinical care and enable novel therapeutic approaches. Our specific aims are 1) Develop enabling technology addressing fundamental limitations in image processing and information fusion, sensing, and manipulation; 2) integrate these components into a modular extendable system to significantly enhance surgeons´ ability to perform microsurgical tasks; and 3) evaluate our systems´ ability to improve surgeon performance of realistic surgical tasks on realistic phantom, cadaver, and animal models associated with three testbed applications. We have chosen surgical treatment of three common sight threatening conditions to provide specific focus for our research pathology of the retinal surface, pathology of the internal limiting membrane, and retinal vein occlusion. The specific capabilities that we propose to develop both address the specific challenges associated with these procedures and are applicable to other techniques and diseases required of vitreoretinal surgery. Their integration will validate our overall system approach and provide a basis for further development both for ophthalmic applications and other microsurgical disciplines such as neurosurgery or microvascular surgery. This Bioengineering Research Partnership (BRP) addresses fundamental limitations in current microsurgical practice, focusing on vitreoretinal surgery, which is the most technically demanding ophthalmologic discipline. The capabilities developed directly address challenges associated with surgical treatment of three of the most common causes of vision loss, which are becoming more prevalent with the aging of our population. Further, these capabilities are broadly applicable in other microsurgical problems, and the system will enable further advances both for ophthalmology and for other microsurgical disciplines

Project start date: 2008-08-01

Project end date: 2013-05-31

1R01EB007969-01A1 (2008): $1183320


Sponsored Links Excellgen http://Excellgen.com

Baculovirus Protein Expression
Fast turn around, >95% purity functional protein. No outsourcing to China or India. $5500, $3950
Recombinant Lentivirus & Adenovirus
High Yield and High Titer virus for Guaranteed Expression of GOI. $3000, $2500
Transient Protein Expression in CHO and HEK293 Cells
Transient Expression, Truly Functional Protein, 95% purity, 1~20 mg, fast turnaround. $5500, $3950