Rebecca M Williams
Cornell University Ithaca
Project start date: 2012-06-15
Project end date: 2013-06-14
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Grants awarded to Rebecca M Williams
EXOCYTOSIS & RECYCLING IN RBL 2H3 MUCOSAL MAST CELLS
Rebecca M Williams
Cornell University Ithaca Office Of Sponsored Programs Ithaca, Ny 148502820
Grant 5P41RR004224-130001 from National Center For Research Resources
Abstract: The mucosal mast cell line RBL-2H3 secrete histamine, serotonin (5-hydroxytryptamine, 5-HT) and other inflammatory mediators in response to applied allergen. Previously it has been shown that 5-HT distributions can be imaged in 5-HT loaded RBL-2H3 cells using three photon excitation (3PE) of 5-HT autofluorescence (Maiti et al., 1997, Science 275530). In this application of multiphoton microscopy, an infrared (740 nm), ultrafast (pulsed) laser was used to excite the < 300 nm 5-HT electronic transition, alleviating chromatic aberrations and the severe photodamage associated with deep UV wavelengths. We are making 3PE imaging measurements of RBL-2H3 cell secretion. During imaging the cells are observed to characteristically ruffle and spread as granular 5-HT autofluorescence disappears with no detectable granule movement. Individual cells exhibit highly heterogeneous release kinetics, often with quasi periodic bursts. Neighboring granule disappearances are generally correlated, indic ative of either spatially localized signaling or granule-granule interactions. After individual g5P41RR004224-130104
Keywords: 2000
Zeiss LSM710 Confocal Microscope For Shared Imaging Facility
Rebecca M Williams
Cornell University Ithaca
Grant 1S10RR025502-01 from National Center For Research Resources IRG: ZRG1
Project start date: 2009-05-14
Project end date: 2010-05-13