Zeiss LMS 510 META NLO Multi-Photon System
Vickie J Lamorte
University Of California Irvine Irvine, Ca 926977600
Grant 1S10RR022612-01 from National Center For Research Resources IRG: ZRG1
Abstract: We are a group of 13 multi-disciplinary investigators representing ten departments at the University of California, Irvine with 9 federally funded research projects impacted by this request for instrumentation. We are seeking funds for a Zeiss LSM 51 0 META NLO multi-photon microscope to be housed and supported within the well-established NIH-NCRR Biomedical Technology Resource Center (P41-RR01192, Laser Microbeam and Medical Program, LAMMP) at the Beckman Laser Institute. The LAMMP facility is dedicated to defining and understanding broad issues related to the use of light as a tool to selectively perturb, monitor, and image physiology in cells and tissues. There is an urgent need for a commercial, user-friendly confocal/multi-photon microscope to be housed and managed within our NIH resource. All of the proposed as pilot/feasibility studies using a non-commercial, home-built multi-photon excitation laser scanning system and/or a 14-year-old LSM 410 confocal system. These projects have expanded in scope, and now have extramural federal support that, in turn, requires frequent and often heavy use of laser scanning microscopy. In order to adequately accommodate the maturation of these innovative studies, we are requesting funds to purchase a Zeiss META instrument. This platform ideally accommodates our needs, which range from low-light-level endogenous signal detection of both fluorescence and second harmonic generation (SHG) in tissues to cellular FRET-based imaging. Although LAMMP facilities include multi-photon and confocal microscopy, these instruments are custom-built, low-throughput devices optimized for special needs and pilot/feasibility data. Consequently, continued progress for these NIH-funded investigators is hampered without access to a commercial-grade instrument. It is expected that the requested instrumentation will have a significant overall benefit to the UCI research community and beyond based on the existing strong impact of the LAMMP and its dissemination of new discoveries to colleagues and collaborators.
Keywords: electromagnetic radiation, laser, housing, lighting, microscopy, tissue
Project start date: 2006-04-15
Project end date: 2007-04-14
1S10RR022612-01 (2006): $500000
Sponsored Links Excellgen http://Excellgen.com
Grants awarded to Vickie J Lamorte
Vickie J Lamorte
Institution:
Grant 3P41RR001192-20S10040 from National Center For Research Resources
Abstract: Transfer of developing LAMMP technologies for optimization of FRET with Sapphire/Topaz pairs.
Project start date: 1999-04-01
Project end date: 2000-03-31
MICROBEAM ABLATION And CELLULAR MECHANISMS
Vickie J Lamorte
University Of California Irvine Irvine, Ca 926977600
Grant 2P41RR001192-190001 from National Center For Research Resources
Abstract: The renaissance of optical microscopy brings with it new capabilities to visualize molecular events at the subcellular level. No longer does microscopy provide the tools only to see the morphology of the cell and its subcomponents, it now serves as a platform for imaging discrete molecular activities with tools to perturb and manipulate these events. Utilization of laser microbeams to inactivate specific cellular components in a living cell and the development of single cell assays to monitor gene expression and other cellular activities to discern function will be highlighted in this section. Our specific aims are to a) Develop in vivo labeling of subcellular structures with green fluorescent protein (GFP) to facilitate microbeam-mediated ablation of an in vivo target and to subsequently assess the resulting structural alteration in the cell, b) Couple laser inactivation with specific cellular/molecular assays in order to elucidate the function of the ablated targets, c) Develop Fluorescence Resonance Energy Transfer (FRET) utilizing green fluorescent protein (GFP)- and blue fluorescent protein (BFP)- fusion proteins and employ FRET as an assay to access the effects of microbeam ablation of discrete subcellular structures or domains of interaction within a cell, d) Combine laser tweezers with sub-ablative microbeams to introduce biological reagents into cells via Ropto-porationS. This will expand the repertoire of microbeam technologies to previously unexplored biological areas i.e. suspension cell models.
Keywords: biological product, biomedical resource, laser, model design /development, technology /technique, human tissue
Project start date: 1998-05-15
Project end date: 1999-03-31
LEF 1 And TCF 1 TRAFFICKING IN LIVING CELLS: RECOMBINANT PROTEIN
Vickie J Lamorte
University Of California Irvine Irvine, Ca 926977600
Grant 3P41RR001192-20S10035 from National Center For Research Resources
Abstract: The purpose of this project would be to monitor LEF-1 and TCF-1 trafficking in living cells. For these experiments, recombinant GST-GFP-LEF-1 and GST-GFP-TCF-1 protein will be microinjected into the cells with an Eppendorf injection system. The injection apparatus will be mounted on a Zeiss microscope platform equipped with a low light level imaging system. The set up will allow for imaging to commence within minutes after injection. Kinetic processes can be resolved by this system down to those occurring within a few seconds.
Keywords: biomedical resource, education
Project start date: 1999-04-01
Project end date: 2000-03-31
PML PROTEIN AS BIOMARKER FOR CERVICAL CANCER: IMMUNOHISTOCHEMISTRY
Vickie J Lamorte
University Of California Irvine Irvine, Ca 926977600
Grant 3P41RR001192-20S10033 from National Center For Research Resources
Abstract: The purpose of this project was to examine PML protein in cervical sections by immunohistochemistry and to establish a correlation between the level of protein expression and the degree of dysplasia.
Keywords: biomedical resource, education
Project start date: 1999-04-01
Project end date: 2000-03-31
SOUTHERN CA CONFOCAL MICROSCOPY USERS GROUP ADVANCED CONFOCAL IMAGING WORKSHOP
Vickie J Lamorte
University Of California Irvine Irvine, Ca 926977600
Grant 5P41RR001192-230036 from National Center For Research Resources
Abstract: The Optical Biology Shared Resource of the Clinical Cancer Center, along with the Laser Microbeam and Medical Program (LAMMP) and BIO-RAD hosted an advanced confocal imaging workshop. There were 100 trainees in attendance.
Keywords: biomedical resource, education
Project start date: 2002-04-01
Project end date: 2003-03-31
Sponsored Links Excellgen http://Excellgen.com
MICROBEAM ABLATION And CELLULAR MECHANISMS: NUCLEAR BODIES
Vickie J Lamorte
University Of California Irvine Irvine, Ca 926977600
Grant 3P41RR001192-20S10001 from National Center For Research Resources
Abstract: Studies examining the role of the PML-containing nuclear bodies in living cells are proposed. As the parameters necessary for ablation of the GFP-labeled bodies are further defined, more fine-tuned experiments are being conducted. Preliminary LAMMP studies also suggest the feasibility of peforming in vivo apoptotic assays on the ablated cells since morphological changes suggest that ablation of all of the PML containing nuclear bodies leads to cell death.
Keywords: biological product, biomedical resource, laser, model design /development, technology /technique, human tissue
Project start date: 1999-04-01
Project end date: 2000-03-31
BIOSCIENCES 199 INDEPENDENT STUDY IN BIOLOGICAL SCIENCES RESEARCH
Vickie J Lamorte
Institution:
Grant 3P41RR001192-20S10044 from National Center For Research Resources
Abstract: Individual experimental laboratory or field research under a professor s direction.
Project start date: 1999-04-01
Project end date: 2000-03-31
FLUORESCENT ANALYSIS OF REGULATION OF CHOLESTEROL METABOLISM IN MAMMALIAN CELLS
Vickie J Lamorte
University Of California Irvine Irvine, Ca 926977600
Grant 5P41RR001192-250045 from National Center For Research Resources IRG: ZRG1
Keywords: Mammalia, bioimaging /biomedical imaging, biomedical resource, cholesterol, fluorescence, imaging /visualization /scanning, steroid metabolism, clinical research
Project start date: 2004-04-01
Project end date: 2005-03-31
PML AS A MARKER FOR CERVICAL CANCER
Vickie J Lamorte
University Of California Irvine Irvine, Ca 926977600
Grant 5P41RR001192-250072 from National Center For Research Resources IRG: ZRG1
Keywords: biomarker, biomedical resource, cervix neoplasm, neoplasm /cancer diagnosis, progressive multifocal leukoencephalopathy, clinical research
Project start date: 2004-04-01
Project end date: 2005-03-31
UPREGULATION OF PML IN CHLAMYDIA INFECTION
Vickie J Lamorte
University Of California Irvine Irvine, Ca 926977600
Grant 5P41RR001192-250073 from National Center For Research Resources IRG: ZRG1
Keywords: biomedical resource, chlamydial disease, progressive multifocal leukoencephalopathy, clinical research
Project start date: 2004-04-01
Project end date: 2005-03-31
SELECTIVE DEACTIVATION OF NUCLEAR BODY, POD, W/ LASER MICROBEAMS
Vickie J Lamorte
University Of California Irvine Irvine, Ca 926977600
Grant 5P41RR001192-180012 from National Center For Research Resources
Keywords: animal tissue, biological product, biomedical resource, biotechnology, genetics, human tissue, laser, neoplasm /cancer, technology /technique development
Project start date: 1997-04-21
Project end date: 1998-03-31