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YEAST GENETIC STOCK CENTER

Shung-chang Jong
American Type Culture Collection
10801 University Blvd
manassas, Va 20110

Grant 5P40RR013278-03 from National Center For Research Resources IRG: RIRG

Abstract: The Yeast Genetic Stock Center (YGSC) at the University of California at Berkeley, established in the early 1960s, includes approximately 1200 singly or multiply-marked, genetically-characterized strains of Saccharomyces cerevisiae. These strains bear one to several mutant loci encompassing a wide range of metabolic functions and requirements and have been widely used for basic and applied research in teaching and research institutes and by industry. The collection´s founder Dr. R.K. Mortimer has selected the American Type Culture Collections (ATCC) as the appropriate place for the transfer and conservation of his collection after retirement. YGSC is currently supported by NCRR/NIH. Many yeast proteins have human homologs. Analysis of the yeast genome will assist in studies of proteins implicated in human heritable diseases, providing an important key to improvements in diagnosis or therapy. In particular, the ease of homologous gene replacement in yeast in yeast really sets this organism apart from other eukaryotic models. ATCC has a history of maintaining and distributing materials in support of the yeast research community. In 1970 the Committee on the Maintenance of Genetic Stocks of the Genetics Society of America requested that ATCC accept certain fungal genetic stocks for cryopreservation. The YGSC collection will be a splendid addition to ATCC´s own growing yeast genetic stock collection and the impetus for developing a yeast genetics research resource center at the ATCC. The YGSC electronic strain data will be included in ATCC´s online catalogs. The Mycology Collection already houses more than 1200 strains of yeasts with genotypes and/or mating type information, hundreds of which are documented transformation hosts. Vectors, which include plasmids, plasmids, phagemids, and YACs suitable for use in yeast hosts, and genetic clones and libraries derived from yeast sources are currently maintained in the Molecular Biology Collection. A set of deletion mutants has been acquired from Dr. J. Boeke of John Hopkins. Dr. M. Johnston of Washington of Washington University has agreed to deposit 6000 knockouts. This proposal requests a one-time grant from NIH for a period of three years to support the transfer of YGSC collection to ATCC, its cryopreservation in liquid nitrogen, and a cost-share for the development of a national genetic resource center, providing a cost-saving, efficient, shared facility for the research community

Keywords: Saccharomyces cerevisiae, cryopreservation, fungal genetics, genetic model, genetic registry /resource /referral center, genetic strain, microorganism resource /registry Internet, artificial chromosome, genome, information dissemination, mutant, transfection /expression vector

Project start date: 1998-09-01

Project end date: 2002-08-31

5P40RR013278-03 (2000): $131956


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YEAST GENETIC STOCK CENTER

Shung-chang Jong
American Type Culture Collection
10801 University Blvd
manassas, Va 20110

Grant 5P40RR013278-02 from National Center For Research Resources IRG: RIRG

Abstract: The Yeast Genetic Stock Center (YGSC) at the University of California at Berkeley, established in the early 1960s, includes approximately 1200 singly or multiply-marked, genetically-characterized strains of Saccharomyces cerevisiae. These strains bear one to several mutant loci encompassing a wide range of metabolic functions and requirements and have been widely used for basic and applied research in teaching and research institutes and by industry. The collection´s founder Dr. R.K. Mortimer has selected the American Type Culture Collections (ATCC) as the appropriate place for the transfer and conservation of his collection after retirement. YGSC is currently supported by NCRR/NIH. Many yeast proteins have human homologs. Analysis of the yeast genome will assist in studies of proteins implicated in human heritable diseases, providing an important key to improvements in diagnosis or therapy. In particular, the ease of homologous gene replacement in yeast in yeast really sets this organism apart from other eukaryotic models. ATCC has a history of maintaining and distributing materials in support of the yeast research community. In 1970 the Committee on the Maintenance of Genetic Stocks of the Genetics Society of America requested that ATCC accept certain fungal genetic stocks for cryopreservation. The YGSC collection will be a splendid addition to ATCC´s own growing yeast genetic stock collection and the impetus for developing a yeast genetics research resource center at the ATCC. The YGSC electronic strain data will be included in ATCC´s online catalogs. The Mycology Collection already houses more than 1200 strains of yeasts with genotypes and/or mating type information, hundreds of which are documented transformation hosts. Vectors, which include plasmids, plasmids, phagemids, and YACs suitable for use in yeast hosts, and genetic clones and libraries derived from yeast sources are currently maintained in the Molecular Biology Collection. A set of deletion mutants has been acquired from Dr. J. Boeke of John Hopkins. Dr. M. Johnston of Washington of Washington University has agreed to deposit 6000 knockouts. This proposal requests a one-time grant from NIH for a period of three years to support the transfer of YGSC collection to ATCC, its cryopreservation in liquid nitrogen, and a cost-share for the development of a national genetic resource center, providing a cost-saving, efficient, shared facility for the research community

Keywords: Saccharomyces cerevisiae, cryopreservation, fungal genetics, genetic model, genetic registry /resource /referral center, genetic strain, microorganism resource /registry Internet, artificial chromosome, genome, information dissemination, mutant, transfection vector

Project start date: 1998-09-01

Project end date: 2001-08-31

5P40RR013278-02 (1999): $118451



Grants awarded to Shung-chang Jong

2ND ANNUAL COMP MEDICINE RESOURCE DIRECTOR´S MEETING

Shung-chang Jong
American Type Culture Collection
10801 University Blvd
manassas, Va 20110

Grant 1U13RR015122-01 from National Center For Research Resources IRG: RIRG

Abstract: The purpose of the NCRR Comparative Medicine Resource Directors´ meeting is to facilitate communication between funded resource centers and NIH staff on important programmatic issues and to enhance information exchange among the individual resources. Both the Resources and NCRR have a mission to provide valuable resources to the biomedical research community and, ultimately, to public health. The first Resource Directors´ meeting was held at the University of Miami National Resource for Aplysia on February 4-5, 1999. The twenty-seven Resource Directors who attended agreed that the meeting was timely and useful and that several subgroups should be formed to address topics from the Working Groups and develop recommendations for the next meeting. As a result of feedback requested by NCRR, the scientific presentations for the upcoming meeting are genetic management, zoonotic diseases, and data management. Genetic management will be the topic for the keynote address and an excellent lead into a small group discussion on this topic. There are two areas that the Resource Directors felt warranted explicit follow-up from the first meeting intellectual property provisions as they affect resource collections and distribution and tracking of resource users and publications. Preparatory working groups for these two areas are being formed to gather information and to work with NCRR staff to determine important discussion points. Before the meeting the two Working Groups will define the issues clearly and pool information about how various resource centers are handling the problems and requirements involved. The proceedings of the meeting will be published and posted as previously on the NCRR website for dissemination among the scientific community at large. This application seeks support for this second annual meeting to be held at the ATCC in Manassas, Virginia on April 27-28, 2000

Keywords: biomedical resource, meeting /conference /symposium data management, genetics, zoonosis

Project start date: 2000-04-01

Project end date: 2002-03-31

1U13RR015122-01 (2000): $39300