IN VIVO IVUS IMAGE-BASED MODELING FOR HUMAN CORONARY PLAQUE ASSESSMENT
Zheng Jie
Worcester Polytechnic Institutecity: Worcester country: United States (us)
Grant 5R01EB004759-07 from National Institute Of Biomedical Imaging And Bioengineering
Keywords: Acute; American Heart Association; Angiography; Arterial Fatty Streak; Automation; base; Benchmarking; Blood flow; Cadaver; Cardiovascular Diseases; Cardiovascular system; Classification; Classification Scheme; clinical application; commercialization; Complement; Computer Simulation; Computer software; Coronary; cost; Data; Data Analyses; Diagnosis; Diagnostic Procedure; Event; Future; Goals; Histology; Human; Hydrogels; Image; Image Analysis; imaging Segmentation; Imaging technology; improved; In Vitro; in vivo; indexing; Lead; Lesion; Liquid substance; Magnetic Resonance Imaging; Measurement; Mechanics; Medicare; Methods; Modeling; Monitor; Morphology; Myocardial Infarction; Patients; Physicians; pressure; Prevention; Procedures; Property; public health medicine (field); public health relevance; Research; Risk; Risk Factors; Rupture; Sampling; Scheme; Screening procedure; Series; shear stress; Signal Transduction; simulation; Site; Stress; Stretching; stroke; Structure; success; Syndrome; Techniques; Testing; three-dimensional modeling; Tissues; tool; Ultrasonography; Validation; validation studies
Relevance: Many cardiovascular events (such as heart attack and stroke) are caused by atherosclerotic plaque rupture which may happen without any warning signals. Success of this project will lead to more accurate in vivo coronary plaque vulnerability assessment and predictions for possible plaque rupture risk so that better and timely decisions for treatment can be made leading to better public health and reduced costs of Medicare. Commercialization of the research results is possible with the automation of model construction and data analysis procedures
Project start date: 2004-09-01
Project end date: 2013-11-30
Budget start date: 1-DEC-2011
Budget end date: 30-NOV-2012
5R01EB004759-07 (2012): $167986
Sponsored Links Excellgen http://Excellgen.com
Grants awarded to Zheng Jie
DEVELOP A DUAL FUNCTIONAL NANOPROBE FOR INTEGRATION OF FLUORESCENCE MICROSCOPY WI
Zheng Jie
University Of Texas Dallascity: Richardson country: United States (us)
Grant 1R21EB011762-01A1 from National Institute Of Biomedical Imaging And Bioengineering
Abstract: The objective of the proposed application is to develop a dual functional probe which is detectable at single-particle level for both Raman and fluorescence microscopy, so that the strengths of each technique can be combined into a single imaging tool for tackling challenges in bioimaging. With this new imaging probe and tool, we plan to address a long-term bioimaging challenge that is real-time imaging of interactions between ligands and receptors at the molecular level inside live cells. Success of this proposed work will (1) provide a new imaging probe that is not only highly fluorescent and robust but can also report molecular information for ligands it labels; (2) offer a new imaging tool which integrates strengths of fluorescence microscopy and Raman spectroscopy; (3) enable us to correlate cellular dynamics of ligands with ligand-binding protein interactions at the chemical level inside live cells. The applications of these fluorescent and Raman probes are not limited to the proposed studies; they can also find applications in bioimaging as multiplexing and multifunctional probes to correlate fluorescence and Raman images with electron microscopy images at in vitro and in vivo level. Ligand-receptor interactions play a pivotal role in regulating cellular functions and provide a foundation for disease diagnosis and new therapy development. However, comprehensive understanding of in vivo interactions between ligand and receptors at the molecular level remains highly challenging. Our research application aims to integrate fluorescence microscopy with Raman spectroscopy using a dual functional probe, so that we can simultaneously track and chemically image of ligand-receptor complexes at the single-cell level inside live cells. Success of this proposed work will (1) provide a new imaging probe that not only is highly fluorescent and robust but also can report molecular information of ligands it labels; (2) offer a new imaging tool which integrate strengths of fluorescence microscopy and Raman spectroscopy; (3) enable us to correlate cellular dynamics of ligands with ligand-receptor interactions at the chemical level inside live cells
Keywords: ing; Address; Affinity; base; Binding (Molecular Function); Binding Proteins; bioimaging; Biology; Cell physiology; Cells; chemical reaction; Chemical Structure; Chemicals; Complex; disease diagnosis; Electron Microscopy; Environment; Event; Fluorescence; Fluorescence Microscopy; Fluorescence Resonance Energy Transfer; Fluorescent Probes; folate-binding protein; Folic Acid; Foundations; Goals; Hand; Homocysteine; Homocystine; Image; Imaging Device; imaging probe; Imaging Techniques; In Situ; In Vitro; in vivo; interest; Label; Life; Ligands; Location; Measures; Medicine; Microscopic; Microscopy; Molecular; nanoparticle; nanoprobe; nanoscience; Nanotechnology; novel therapeutics; Nucleotide Biosynthesis; particle; Play; programs; protein complex; Proteins; Raman Spectrum Analysis; Reaction; receptor; receptor binding; Reporting; Research; Research Proposals; Resolution; response; Role; Signal Transduction; Silver; solid state; Spectrum Analysis; Structure; success; Surface; Techniques; therapeutic development; therapy development; Time; tool; Work; X-Ray Crystallography
Relevance: Ligand-receptor interactions play a pivotal role in regulating cellular functions and provide a foundation for disease diagnosis and new therapy development. However, comprehensive understanding of in vivo interactions between ligand and receptors at the molecular level remains highly challenging. Our research application aims to integrate fluorescence microscopy with Raman spectroscopy using a dual functional probe, so that we can simultaneously track and chemically image of ligand-receptor complexes at the single-cell level inside live cells. Success of this proposed work will (1) provide a new imaging probe that not only is highly fluorescent and robust but also can report molecular information of ligands it labels; (2) offer a new imaging tool which integrate strengths of fluorescence microscopy and Raman spectroscopy; (3) enable us to correlate cellular dynamics of ligands with ligand-receptor interactions at the chemical level inside live cells
Project start date: 2011-07-01
Project end date: 2013-06-30
Budget start date: 1-JUL-2011
Budget end date: 30-JUN-2012
PFA/PA: PA-08-053
1R21EB011762-01A1 (2011): $210420