RNA BINDING PROTEINS AND SPERMATOGENESIS
Robert E Braun, Associate Professor
University Of Washington Office Of Sponsored Programs Seattle, Wa 98105
Grant 5R01HD027215-09 from National Institute Of Child Health And Human Development IRG: REB
Abstract: Determining the events associated with the processing, transport, localization, storage, and translation of a mRNA are critical to our overall understanding of when, where, and how much of a protein is synthesized. In recent years the pivotal role of the 3 untranslated region (3 UTR) of the mRNA in affecting these facets of mRNA behavior has been amply demonstrated. For example, during mammalian spermatogenesis, the Prm-1 gene is first transcribed shortly after meiosis in round spermatids, however, its mRNA is not translated until up to a week later in elongating spermatids. The study of fusion genes in transgenic mice has shown that the cis-acting sequence elements required for Prm-1 translational repression map within the 3 UTR of the mRNA. The next advance in the understanding of how 3 UTRs mediate their diverse effects on mRNA will require the identification of the trans-acting factors that interact with the 3 UTRs and that link them to other molecules in the cell. The broad goals of our research are to identify genes involved in controlling the various aspects of mRNA behavior described above, and to investigate the mechanisms by which they function during mammalian germ cell development. Specifically, in this proposal we intend to 1) Determine the consequences of premature translation of Prm-1 mRNA during the early haploid stages of spermatogenesis in transgenic mice. 2) Clone the gene for a germ cell-specific protein that binds to the 3 UTRs of the mouse Prm-1 and Prm-2 mRNAs. 3) Create and analyze a mutation in the Prbp gene in transgenic mice. Create a series of mutations in the Prm-1 3 UTR and assay them for Prbp binding in vitro. Create and analyze two Prbp binding site-mutations in vivo. Knowledge gained from these studies may ultimately contribute to our understanding of some causes of male infertility, to the inheritance of genetic diseases, and to the development of a male contraceptive.
Keywords: RNA binding protein, developmental genetics, genetic translation, germ cell, protamine, spermatogenesis, genetic regulatory element, mammalian embryology, messenger RNA, molecular site, sperm, transcription factor, embryonic stem cell, fusion gene, gene mutation, gene targeting, immunocytochemistry, in situ hybridization, laboratory mouse, monoclonal antibody, northern blotting, polymerase chain reaction, protein purification, transgenic animal, western blotting
Project start date: 1990-09-01
Project end date: 2000-01-31
5R01HD027215-09 (1999): $199693
Sponsored Links Excellgen http://Excellgen.com
Translational Control During Murine Spermatogenesis
Robert E Braun, Associate Professor
Jackson Laboratory
Grant 5R01HD027215-19 from Eunice Kennedy Shriver National Institute Of Child Health & Human Development IRG: CMIR
Abstract: The production of gametes in mammals is an elaborate process that begins during embryogenesis and continues during the reproductive life of the organism. Gametogenesis involves the production of highly specialized cells with unique organelles designed to accomplish the union of sperm and egg at fertilization. The timely production of developmental^ important products involves the regulated translation of mRNAs that have accumulated earlier during gametogenesis. Deviation from the wild-type timing of translation can cause cessation of gametogenesis and lead to sterility. The goals of this proposal are to further our understanding of the mechanisms of translational repression and activation during mammalian spermatogenesis. The studies in this proposal will focus on the regulation of posttranscriptional control in adult male germ line cells in mice. In Aim 1, the function of the Y box proteins MSY2 and MSY4 will be investigated using conditional gene targeting. In Aim 2, MSY2 and MSY4-interacting proteins will be identified by mass spectrometry analysis of ribonucleoprotein particles precipitated with MSY2 and MSY4 antibodies. Prm1-enriched mRNPs will be isolated using biotinylated antisense RNAs and streptavidin-coated immunomagnetic beads. In Aim 3, proteins identified by mass spectrometry will be verified using complementary methods and functional studies will be pursued in cell culture and in vitro to determine how the associated proteins contribute to translational repression and mRNA stability. The knowledge gained from these studies may be useful in the genetic assessment of male infertility in humans, and lead to the development of male contraceptives designed to disrupt essential regulatory steps during normal spermatogenesis
Project start date: 1990-09-01
Project end date: 2011-02-28
5R01HD027215-16 (2007): $313894
5R01HD027215-14 (2004): $303816
5R01HD027215-13 (2003): $294965
5R01HD027215-12 (2002): $286371
5R01HD027215-11 (2001): $278031
TRANSLATIONAL REGULATION OF THE PROTAMINE 1 GENE
Robert E Braun, Associate Professor
University Of Washington Office Of Sponsored Programs Seattle, Wa 98105
Grant 5R01HD027215-05 from National Institute Of Child Health And Human Development IRG: REB
Abstract: One major goal of this proposal is to determine the molecular mechanism of temporal translational control during male germ cell development in the mouse. A second major objective is to investigate the role of the protamine 1 gene in the physical remodelling and the genetic reprogramming of the paternal genome during spermatogenesis. The mouse protamine 1 gene, Prm-1, is regulated at both the transcriptional and translational level. Although the gene is first transcribed postmeiotically in round spermatids, the mRNA is not translated until up to a week later in elongating spermatids. To identify the cis-acting regulatory elements in the Prm-1 mRNA that regulate its temporal translational control, fusions will be constructed between regions of the Prm-1 gene and a reporter gene and studied in transgenic mice. Cis-acting translational control elements that are identified will be cloned into different regions of an heterologous mRNA to determine if their position within an mRNA affects their function. To study the mechanism of translational control, genetic and biochemical approaches will be used to clone the gene(s) for translational control factor(s) that interact with the translational control elements. To determine the importance of temporal translational control as it pertains to protamine 1 function, the product of the Prm-1 gene will be expressed at different times during spermatogenesis. To determine if protamine 1 is essential for nuclear condensation and/or reprogramming of the paternal genome during spermatogenesis, the gene will be mutated in embryonic stem cells by homologous recombination and its effect studied in transgenic mice. Knowledge gained from these studies may ultimately contribute to the medically important problems of male infertility and the inheritance of genetic diseases.
Keywords: cell nucleus, genetic translation, germ cell, protamine, spermatogenesis, RNA binding protein, early embryonic stage, fusion gene, genetic enhancer element, genetic manipulation, genetic recombination, histogenesis, mammalian embryology, molecular cloning, morphology, sperm, testis, embryonic stem cell, gel electrophoresis, genetic mapping, immunocytochemistry, in situ hybridization, laboratory mouse, laboratory rabbit, male, polymerase chain reaction, reporter gene, tissue /cell culture, transgenic animal
Project start date: 1990-09-01
Project end date: 1996-01-31
5R01HD027215-05 (1994): $113121
5R01HD027215-04 (1993): $108627
TRANSLATIONAL REGULATION OF THE MOUSE PROTAMINE 1 GENE
Robert E Braun, Associate Professor
University Of Washington Office Of Sponsored Programs Seattle, Wa 98105
Grant 5R01HD027215-03 from National Institute Of Child Health And Human Development IRG: REB
Abstract: One major goal of this proposal is to determine the molecular mechanism of temporal translational control during male germ cell development in the mouse. A second major objective is to investigate the role of the protamine 1 gene in the physical remodelling and the genetic reprogramming of the paternal genome during spermatogenesis. The mouse protamine 1 gene, Prm-1, is regulated at both the transcriptional and translational level. Although the gene is first transcribed postmeiotically in round spermatids, the mRNA is not translated until up to a week later in elongating spermatids. To identify the cis-acting regulatory elements in the Prm-1 mRNA that regulate its temporal translational control, fusions will be constructed between regions of the Prm-1 gene and a reporter gene and studied in transgenic mice. Cis-acting translational control elements that are identified will be cloned into different regions of an heterologous mRNA to determine if their position within an mRNA affects their function. To study the mechanism of translational control, genetic and biochemical approaches will be used to clone the gene(s) for translational control factor(s) that interact with the translational control elements. To determine the importance of temporal translational control as it pertains to protamine 1 function, the product of the Prm-1 gene will be expressed at different times during spermatogenesis. To determine if protamine 1 is essential for nuclear condensation and/or reprogramming of the paternal genome during spermatogenesis, the gene will be mutated in embryonic stem cells by homologous recombination and its effect studied in transgenic mice. Knowledge gained from these studies may ultimately contribute to the medically important problems of male infertility and the inheritance of genetic diseases.
Keywords: cell nucleus, genetic translation, germ cell, protamine, spermatogenesis, early embryonic stage, fusion gene, genetic enhancer element, genetic manipulation, genetic recombination, histogenesis, mRNA binding protein, mammalian embryology, molecular cloning, morphology, sperm, testis, embryonic stem cell, gel electrophoresis, genetic mapping, immunocytochemistry, in situ hybridization, laboratory mouse, laboratory rabbit, male, polymerase chain reaction, reporter gene, tissue /cell culture, transgenic animal
Project start date: 1990-09-01
Project end date: 1995-04-30
5R01HD027215-03 (1992): $106294
Sponsored Links Excellgen http://Excellgen.com
RNA BINDING PROTEINS AND SPERMATOGENESIS
Robert E Braun, Associate Professor
University Of Washington Office Of Sponsored Programs Seattle, Wa 98105
Grant 5R01HD027215-08 from National Institute Of Child Health And Human Development IRG: REB
Abstract: Determining the events associated with the processing, transport, localization, storage, and translation of a mRNA are critical to our overall understanding of when, where, and how much of a protein is synthesized. In recent years the pivotal role of the 3 untranslated region (3 UTR) of the mRNA in affecting these facets of mRNA behavior has been amply demonstrated. For example, during mammalian spermatogenesis, the Prm-1 gene is first transcribed shortly after meiosis in round spermatids, however, its mRNA is not translated until up to a week later in elongating spermatids. The study of fusion genes in transgenic mice has shown that the cis-acting sequence elements required for Prm-1 translational repression map within the 3 UTR of the mRNA. The next advance in the understanding of how 3 UTRs mediate their diverse effects on mRNA will require the identification of the trans-acting factors that interact with the 3 UTRs and that link them to other molecules in the cell. The broad goals of our research are to identify genes involved in controlling the various aspects of mRNA behavior described above, and to investigate the mechanisms by which they function during mammalian germ cell development. Specifically, in this proposal we intend to 1) Determine the consequences of premature translation of Prm-1 mRNA during the early haploid stages of spermatogenesis in transgenic mice. 2) Clone the gene for a germ cell-specific protein that binds to the 3 UTRs of the mouse Prm-1 and Prm-2 mRNAs. 3) Create and analyze a mutation in the Prbp gene in transgenic mice. Create a series of mutations in the Prm-1 3 UTR and assay them for Prbp binding in vitro. Create and analyze two Prbp binding site-mutations in vivo. Knowledge gained from these studies may ultimately contribute to our understanding of some causes of male infertility, to the inheritance of genetic diseases, and to the development of a male contraceptive.
Keywords: RNA binding protein, developmental genetics, genetic translation, germ cell, protamine, spermatogenesis, genetic regulatory element, mammalian embryology, messenger RNA, molecular site, sperm, transcription factor, embryonic stem cell, fusion gene, gene mutation, gene targeting, immunocytochemistry, in situ hybridization, laboratory mouse, monoclonal antibody, northern blotting, polymerase chain reaction, protein purification, transgenic animal, western blotting
Project start date: 1990-09-01
Project end date: 2000-01-31
5R01HD027215-08 (1998): $227963
5R01HD027215-07 (1997): $181441
Grants awarded to Robert E Braun
Program For Fellows And New Investigators
Robert E Braun, Associate Professor
University Of Washington Office Of Sponsored Programs Seattle, Wa 98105
Grant 1U54HD042454-010005 from National Institute Of Child Health And Human Development IRG: ZHD1
Abstract: We intend to create a program to attract new fellows (M.D. and Ph.D.) for research and training in basic and clinical reproductive biology and to provide research support to outstanding investigators outside the area of contraception development to entice them into working on contraception. We believe there is currently a shortage in the number of new investigators entering the field of reproduction research, especially as it relates to contraceptive research and development. We hope to attract three types of individuals to our Center. The first are outstanding Ph.D. trained fellows who have achieved significant accomplishments in research areas within and outside of mammalian reproduction. The goal is to attract creative young scientists with original ideas. In some cases, these fellows will have been trained as graduate students in reproduction research. However, we especially want to attract fellows who have received graduate training in research areas outside of reproduction. We believe these fellows will bring fresh outlooks and will have a high chance of success in making the transition to independent investigators. Our second group of fellows will be those trained as medical doctors or as physician scientists. Medical fellows will be recruited from residency and fellowship programs in obstetrics and gynecology, reproductive endocrinology, medicine and urology. We believe it is important to attract medical fellows to the Center, as they should be better trained in the practical considerations related to translating research in contraceptive development from basic research to studies in humans and eventually into the practice of medicine. Fellows will be recruited both locally and nationally. Lastly, we will provide research funds for 1-2 year pilot projects to new investigators in reproduction research. New investigators are defined as researchers at any stage in their career that are new to the field of reproduction and who are proposing research related to contraception. This includes new Assistant Professors just starting their labs and established Full Professors who have not previously worked in the field of contraception but who are interested in initiating new studies in this field. We fully expect that these pilot projects will translate into preliminary data for new R01 proposals from these individuals.
Keywords: health science research, postdoctoral investigator, reproduction, training, contraceptive, cooperative study, health science research potential
Project start date: 2002-09-11
Project end date: 2007-02-28
Clinical And Basic Studies In Male Reproduction
Robert E Braun, Associate Professor
University Of Washington Office Of Sponsored Programs Seattle, Wa 98105
Grant 2U54HD012629-27 from National Institute Of Child Health And Human Development IRG: ZHD1
Abstract: This proposal is designed to increase our knowledge and understanding of reproductive biology and medicine in the male. This is a multidisciplinary and collaborative effort among basic scientists and clinical investigators, who are committed to advancing our understanding of reproductive physiology and translate this knowledge into improving human health and well-being. The proposed aims comprise the three major branches of the reproductive axis- the hypothalamus, pituitary and testes. The proposed research embraces both basic and clinical investigations, which have direct relevance to understanding certain disorders of human reproduction, including idiopathic hypogonadotropic hypogonadism, androgen insensitivity syndrome, meiotic arrest, Sertoli cell only syndrome, and oligospermia or azoospermia. Our clinical project is focused on understanding the functional significance of the hormonal and genetic environment required for spermatogenesis in men. The basic science projects investigate the neuroendocrine control of gonadotropin secretion, the molecular basis of the blood-testes barrier, and the key signaling pathways that regulate the development and function of mature gametes. Each project involves collaboration with at least one other in the Center, and the molecular physiology is integrated with the clinical investigation. We believe this approach will offer the best opportunity to provide the scientific rationale for the development of improved therapies to treat certain disorders of reproduction and serve as the basis for the development of better options for hormonal contraception in men. We are committed to scientific excellence and communication across disciplines, ranging from molecular biology to physiology and medicine. Our proposal incorporates the talents of outstanding investigators, whose experience and knowledge represent a rich and broad perspective on reproductive biology and its relevance to human health. We have deliberately structured the Center to bring together the best and most accomplished reproductive biologists and physician-scientists at the University of Washington in a scientific collaboration. We look forward to our continued participation in the NIH Specialized Cooperative Centers Program in Reproductive Research (SCCPRR). We share its goal of fostering communication, innovation and research excellence, with the ultimate goal of improving human reproductive health through accelerated transfer of discoveries made at the bench into clinical practice. We are committed to the collaborative spirit of the SCCPRR and have established collaborations with investigators at SCCPRR Programs at several other institutions. We look forward to contributing to the outstanding clinical and basic research in this important national program.
Project start date: 1997-04-01
Project end date: 2011-03-31
2U54HD012629-27 (2006): $1697334
Translational Control During Murine Spermatogenesis
Robert E Braun, Associate Professor
Jackson Laboratory
Grant 7R01HD027215-17 from National Institute Of Child Health And Human Development IRG: CMIR
Abstract: The production of gametes in mammals is an elaborate process that begins during embryogenesis and continues during the reproductive life of the organism. Gametogenesis involves the production of highly specialized cells with unique organelles designed to accomplish the union of sperm and egg at fertilization. The timely production of developmental^ important products involves the regulated translation of mRNAs that have accumulated earlier during gametogenesis. Deviation from the wild-type timing of translation can cause cessation of gametogenesis and lead to sterility. The goals of this proposal are to further our understanding of the mechanisms of translational repression and activation during mammalian spermatogenesis. The studies in this proposal will focus on the regulation of posttranscriptional control in adult male germ line cells in mice. In Aim 1, the function of the Y box proteins MSY2 and MSY4 will be investigated using conditional gene targeting. In Aim 2, MSY2 and MSY4-interacting proteins will be identified by mass spectrometry analysis of ribonucleoprotein particles precipitated with MSY2 and MSY4 antibodies. Prm1-enriched mRNPs will be isolated using biotinylated antisense RNAs and streptavidin-coated immunomagnetic beads. In Aim 3, proteins identified by mass spectrometry will be verified using complementary methods and functional studies will be pursued in cell culture and in vitro to determine how the associated proteins contribute to translational repression and mRNA stability. The knowledge gained from these studies may be useful in the genetic assessment of male infertility in humans, and lead to the development of male contraceptives designed to disrupt essential regulatory steps during normal spermatogenesis
Keywords: protein, spermatogenesis Alphaherpesvirinae, RNA binding protein, antibody, binding protein, biotin, cell, cell line, contraceptive, embryogenesis, embryonic stem cell, fertility, fertilization, gametogenesis, gene, gene mutation, gene targeting, genetics, germ cell, human, lead, male, mass spectrometry, motivation, mutant, organelle, organism, particle, precipitation, protamine, protein binding, repression, ribonucleoprotein, role, spectrometry, sperm, thymidine kinase, tissue /cell culture, transfection
Project start date: 1990-09-01
Project end date: 2011-02-28
2R01HD027215-15A1 (2006): $322663
2R01HD027215-10 (2000): $269933
Spermatogonial Stem Cell Renewal And Differentiation
Robert E Braun, Associate Professor
University Of Washington Office Of Sponsored Programs Seattle, Wa 98105
Grant 1U54HD042454-010002 from National Institute Of Child Health And Human Development IRG: ZHD1
Abstract: Continuous spermatogenesis in a normal individual requires maintenance of a pool of spermatogonial stem cells. These cells, which are the precursors to all mature spermatozoa, must achieve a balance between differentiation and self-renewal. Our current knowledge that stem cells lie outside of the blood testis barrier, that maintenance of the stem cell pool requires signaling between somatic cells and germ cells, and that the balance between stem cell renewal and differentiation is regulated, suggest it might be feasible to develop contraceptive agents that reversibly block the initiation of spermatogenesis. We propose to increase the basic knowledge of spermatogonial stem cell renewal and differentiation by studying the luxoid mutation. The luxoid mutant arose spontaneously in 1950 and was placed in cryostorage in 1970. We have had this mutant revived from cryostorage and shown that it causes a depletion of spermatogonial stem cells. Luxoid appears to cause the same phenotype as a mutation in the glial-derived neurotrophic factor (Gdnf) gene, the only other mutation known to affect the balance between spermatogonial stem cell renewal and differentiation. We propose to clone the gene that is responsible for the luxoid phenotype, assess whether the gene is required in the somatic or germ cell compartment of the testis, and determine if luxoid functions in the same genetic pathway as Gdnf. Our immediate studies will expand our understanding of the genetic regulation of spermatogonial stem cell renewal. Knowledge gained from our studies will shed light on the feasibility of developing contraceptive strategies aimed at blocking the initiation of spermatogenesis.
Keywords: cell differentiation, cell growth regulation, fertility, genetic regulation, sperm, stem cell, aging, apoptosis, cell cell interaction, cell population study, cooperative study, gene expression, gene mutation, phenotype, spermatogenesis, gene targeting, genetic mapping, immature animal, immunocytochemistry, laboratory mouse, male, mature animal, molecular cloning, terminal nick end labeling, transgenic animal
Project start date: 2002-09-11
Project end date: 2007-02-28
CONFERENCE ON GERM CELL DIFFERENTIATION
Robert E Braun, Associate Professor
Keystone Center Box 8606, 0175 Summit Co Rd Dillon, Co 80435
Grant 1R13HD035068-01 from National Institute Of Child Health And Human Development IRG: CHHD
Abstract: Adapted from applicant s description) Cells of the germline are potentially immortal and carry the genome from generation to generation. Analyses of germ cell development, growth and differentiation in a variety of species has begun to reveal some of the regulatory hierarchies that control these processes. Segregated from the somatic cell lineages during embryonic development, germ cells must retain (or regain) developmental totipotency to give rise to an egg cell that is totiposperm. What distinguishes a germ cell from a somatic cell? How is totipotency regulated in the germline? What factors regulate meiosis and genetic recombinations? How are the processes of X chromosome inactivation and imprinting regulated in germ cells? How can the germline be manipulated? The meeting will address these and other key questions by comparing and contrasting research in a number of experimental organisms.
Keywords: cell differentiation, germ cell, meeting /conference /symposium, travel
Project start date: 1997-03-15
Project end date: 1998-02-28
1R13HD035068-01 (1997): $6000
Robert E Braun
Jackson Laboratory
Project start date: 2011-09-01
Project end date: 2016-08-31
PROJECT 1 - CLINICAL AND BASIC STUDIES IN MALE REPRODUCTION
Robert E Braun, Assoc Director/chair Research
University Of Washington, Office Of Sponsored Programs, Seattle, Wa 98195-9472
Grant 3U54HD012629-30S1_7090 from Eunice Kennedy Shriver National Institute Of Child Health & Human Development
Keywords: (17Beta)-17-hydroxyandrost-4-en-3-one; 17-beta-Hydroxy-4-Androsten-3-one; 1H-Thieno(3, 4-d)imidazole-4-pentanoic acid, hexahydro-2-oxo-, (3aS-(3aalpha, 4beta, 6aalpha))-; 2-Naphthacenecarboxamide, 4-(dimethylamino)-1, 4, 4a, 5, 5a, 6, 11, 12a-octahydro-3, 5, 10, 12, 12a-pentahydroxy-6-methyl-1, 11-dioxo-, (4S-(4alpha, 4aalpha, 5alpha, 5aalpha, 6alpha, 12aalpha))-; ATGN; Ablation; Address; Adherens Junction; Adhering Junction; Adhesive Junction; Anchoring Junction; Androgen Receptor; Androgenic Agents; Androgenic Compounds; Androgens; Androst-4-en-17beta-ol-3-one; Antigens; Apoptosis; Apoptosis Pathway; Area; Assay; Automobile Driving; Bioassay; Biologic Assays; Biological Assay; Biotin; Blood-Testis Barrier; Cannot achieve a pregnancy; Cell Death, Programmed; Cells; Chemotherapy-Hormones/Steroids; Clinical; Contraceptive Agents; Contraceptives; Defect; Delta4-androsten-17beta-ol-3-one; Difficulty conceiving; Down-Regulation; Down-Regulation (Physiology); Downregulation; Doxycycline; Drivings, Automobile; Electron Microscopy; Endocrine Gland Secretion; Environment; Fecundability; Fecundity; Fertility; Gametes; Gene Targeting; Genes; Genetic; Genital System, Male, Testis; Germ Cells; Germ-Line Cells; Hormones; Immune; Infertility; Integral Membrane Protein; Interstitial Cell of Leydig; Interstitial Cell of the Testis; Intrinsic Membrane Protein; Knock-out; Knockout; Knowledge; Lead; Leydig Cells; Light; Liquid substance; Maintenance; Maintenances; Mammalia; Mammals; Mammals, General; Mammals, Mice; Measures; Mice; Molecular; Murine; Mus; Occluding Junctions; Pb element; Permeability; Photoradiation; Property; Property, LOINC Axis 2; Regulation; Reproduction; Reproductive Cells; Research; Resistance; Seminiferous Epithelium; Seminiferous Tubules; Seminiferous tubule structure; Sertoli Cells; Sex Cell; Spermatocytes; Spermatogenesis; Spermiocytes; Staging; Structure of interstitial cell of Leydig; Structure of sertoli cell; Structure of spermatogenic epithelium; Sustentacular Cell of Testis; T-Cells; T-Lymphocyte; Tag; Targetings, Gene; Testicles; Testicular Interstitial Cells; Testing; Testis; Testosterone; Therapeutic Androgen; Therapeutic Hormone; Therapeutic Testosterone; Thymus-Dependent Lymphocytes; Tight Junctions; Time; Tracer; Trans-Testosterone; Transgenes; Transmembrane Protein; Vibramycin; Vitamin H; Work; Zonula Occludens; alpha-6-Deoxyoxytetracycline; base; claudin 3; coenzyme R; driving; experiment; experimental research; experimental study; fluid; heavy metal Pb; heavy metal lead; immunogen; in vivo; infertile; initial cell; leydig interstitial cell; liquid; male; men; men`s; mutant; receptor expression; receptor function; research study; resistant; sertoli cell; sexual cell; small molecule; spermatogenic epithelium structure; thymus derived lymphocyte; unable to bear children
Relevance: The mechanism of androgen regulation of spermatogenesis is of fundamental importance to both infertility and contraceptive research. It is our hope that successful completion of these studies will significantly advance our knowledge in these two areas of male reproduction
Project start date: 2009-04-01
Project end date: 2011-03-31
Budget start date: 1-APR-2009
Budget end date: 31-MAR-2010
3U54HD012629-30S1_7090 (2009): $348000
THE MAMMALIAN REPRODUCTIVE GENETIC DATABASE
Robert E Braun, Assoc Director/chair Research
University Of Washington, Office Of Sponsored Programs, Seattle, Wa 98195-9472
Grant 5U54HD012629-30_9005 from Eunice Kennedy Shriver National Institute Of Child Health & Human Development
Keywords: 21+ years old; ATP[{..}]protein-tyrosine O-phosphotransferase; Adult; Age; Androgenic Agents; Androgenic Compounds; Androgens; Animals; Antibodies; Area; Body Tissues; Cannot achieve a pregnancy; Cells; Chemotherapy-Hormones/Steroids; Clinical; Comment; Comment (PT); Comment [Publication Type]; Commentary; Commentary (PT); Communities; Data; Data Banks; Data Bases; Data Set; Databank, Electronic; Databanks; Database, Electronic; Databases; Databases, Genetic; Dataset; Deposit; Deposition; Development; Difficulty conceiving; E-Mail; EPH- and ELK-Related Tyrosine Kinase; EPH-and ELK-Related Kinase; EPHA8; Editorial Comment; Editorial Comment (PT); Electronic Mail; Email; Embryo; Embryonic; Endocrine Gland Secretion; EphA8 Protein; Ephrin Type-A Receptor 8; Ephrin Type-A Receptor 8 Precursor; Expression Profiling; Expression Signature; Female; Fixation; Frequencies (time pattern); Frequency; Future; Genbank; Gene Transcription; Genes; Genetic; Genetic Alteration; Genetic Change; Genetic Data Banks; Genetic Data Bases; Genetic Databanks; Genetic Databases; Genetic Information Databases; Genetic Transcription; Genetic defect; Genital System, Male, Testis; Genomics; HEK3; Hormones; Human, Adult; Image; Individual; Infertility; Informatics; Investigators; Journals; Laboratories; Letters; Link; Magazine; Mails; Mammals, Mice; Methods; Mice; Microarray Analysis; Microarray-Based Analysis; Modeling; Molecular Fingerprinting; Molecular Profiling; Murine; Mus; Mutation; Names; Neuroendocrine; Neuroendocrine System; Neurosecretory Systems; Oogenesis; PTK; Pathway interactions; Phenotype; Printing; Programs (PT); Programs [Publication Type]; Protein Tyrosine Kinase; Protein Tyrosine Kinase EEK; Proteins; PubMed; Published Comment; Publishing; RNA Expression; Reproduction; Reproductive Biology; Research; Research Personnel; Research Resources; Researchers; Resources; Scientist; Sertoli Cells; Site; Spermatids; Spermatoblasts; Spermatocytes; Spermatogenesis; Spermatogonia; Spermatophores; Spermiocytes; Staging; Staining method; Stainings; Stains; Structure of sertoli cell; Sustentacular Cell of Testis; Swiss Prot; SwissProt; TXT; Testicles; Testis; Text; Therapeutic Androgen; Therapeutic Hormone; Time; Tissues; Transcription; Transcription, Genetic; Tyrosine Kinase; Tyrosine-Protein Kinase Receptor EEK; Tyrosine-Specific Protein Kinase; Tyrosylprotein Kinase; Veins; Viewpoint; Viewpoint (PT); Writing; ing; adult human (21+); cell type; clinical data repository; clinical data warehouse; data repository; design; designing; experiment; experimental research; experimental study; gene product; genome mutation; genome, mouse; hydroxyaryl protein kinase; imaging; improved; infertile; interest; male; meetings; member; microarray technology; molecuar profile; molecular signature; mouse genome; pathway; pituitary gonadal axis; postnatal; programs; relational database; reproductive; research study; sample fixation; sertoli cell; spermatogone; spermatospore; spermid; spermigonium; spermoblast; spermospore; tool; tyrosyl protein kinase; unable to bear children; web page; web site
Budget start date: 1-APR-2009
Budget end date: 31-MAR-2010
PFA/PA: RFA-HD-04-030
5U54HD012629-30_9005 (2009): $213252
Sponsored Links Excellgen http://Excellgen.com
Spermatogonial Stem Cell Self-Renewal And Differentiation
Robert E Braun, Associate Professor
University Of Washington
office Of Sponsored Programs
seattle, Wa 98105
Grant 5U54HD042454-070002 from National Institute Of Child Health And Human Development IRG: ZHD1
Robert E Braun, Associate Professor
University Of Washington
office Of Sponsored Programs
seattle, Wa 98105
Grant 5U54HD042454-070006 from National Institute Of Child Health And Human Development IRG: ZHD1
MEIOTIC CHROMOSOMAL PROTEIN FUNCTION
Robert E Braun, Associate Professor
University Of Washington Office Of Sponsored Programs Seattle, Wa 98105
Grant 5P50HD012629-180012 from National Institute Of Child Health And Human Development
Keywords: cell cycle, cell cycle protein, meiosis, protein structure /function, spermatogenesis, DNA binding protein, RNA binding protein, genetic mapping, genetic transcription, ovary, testis, chimeric protein, confocal scanning microscopy, gene targeting, laboratory mouse, nucleic acid hybridization, nucleic acid sequence, tissue /cell culture
Project start date: 1997-04-01
Project end date: 1998-03-31
RNA BINDING PROTEINS AND SPERMATOGENESIS
Robert E Braun, Associate Professor
Geneticsuniversity Of Washington
office Of Sponsored Programs
seattle, Wa 981959472
Grant 2R01HD027215-06A1 from Eunice Kennedy Shriver National Institute Of Child Health & Human Development IRG: REB
Project start date: 1990-09-01
Project end date: 2000-01-31
2R01HD027215-06A1 (1996): $131752
MEIOTIC CHROMOSOMAL PROTEIN FUNCTION
Robert E Braun, Associate Professor
Institution:
Grant 2P50HD012629-170012 from National Institute Of Child Health And Human Development
Keywords: cell cycle, cell cycle protein, meiosis, protein structure function, spermatogenesis, DNA binding protein, RNA binding protein, genetic mapping, genetic transcription, ovary, testis, chimeric protein, confocal scanning microscopy, gene targeting, laboratory mouse, nucleic acid hybridization, nucleic acid sequence, tissue /cell culture