Protein Production
293FT, 293E, CHO

Truly Functional Protein
95% Purity
1-10 mg in 2 weeks

GeneExpressoMax™
293Expresso™

Transfection Reagents
* 90% Efficiency
* 95% Viability
* No sera interference
* Simple protocol
* High-throughput
* Only $98/ml

Baculovirus
Functional Protein
95% Purity
Fast turnaround
1-10 mg from Sf9 cells

Adenovirus, AAV
& Lentivirus

ORF or shRNA
* High Titer
* Cre, FLP, ΦC31
* Protein Kinases
* Transcription Factors
* Luciferases, GFP, RFP
* Protein Production
* Stable Cell Line


Excellgen

LONG TERM EFFECTS OF PREMARTIAL INTERVENTION

Howard J Markm
University Of Denver, Office Of Sponsored Programs, Denver, Co 80208

Grant 5R01HD053314-23 from Eunice Kennedy Shriver National Institute Of Child Health & Human Development

Abstract: The overarching aim of this proposal is to continue a large-scale community based prevention trial of a marriage education program (PREP, Prevention and Relationship Enhancement Program) delivered in Religious Organizations (ROs) to premarital couples. This program is designed to lower risk factors and raise protective factors for marital distress and associated mental health problems. Given the strong links between marital distress and mental disorders, efforts to prevent mental disorder due to marital conflict has substantial advantages over later and more costly treatment. Targeting ROs as a delivery system is important as ROs already serve as a less stigmatizing resource for prevention and counseling for many people who will not seek services from a mental health professional. This work assesses the value of preventing (vs. treating) mental disorders through maximizing functioning during a critical adulthood transition (i.e., entering marriage). This work proposes to follow an existing sample of 217 couples into a high risk period for distress and divorce (years 5-10 of marriage) in order to assess the long-term/durable preventative effects of premarital intervention on marital distress and symptoms of mental disorders. We will also test and refine our theoretical model of how targeted risk (e.g., negative interactions) and protective factors (e.g., confidence in handling conflicts) are linked over time to marital distress and mental disorder. Benefits include enhancing existing "natural" interventions in the community (premarital counseling) and of reaching a population who might not seek services through the traditional mental health system (e.g., men, minority group members)

Keywords: Communities; Community based prevention; Conflict; Conflict (Psychology); Counseling; Couples; Distress; Divorce; Education; Educational aspects; Effects, Longterm; Ethnic and Racial Minorities; Health Care Professional; Health Professional; Health profession; Health system; Healthcare professional; Healthcare worker; Intervention; Intervention Strategies; Link; Long-Term Effects; Marriage; Mental Health; Mental Hygiene; Mental disorders; Mental health disorders; Minority Groups; Models, Theoretic; Population; Prevention; Programs (PT); Programs [Publication Type]; Psychiatric Disease; Psychiatric Disorder; Psychological Health; Religion and Spirituality; Research Resources; Resources; Risk; Risk Factors; Sampling; Services; Symptoms; System; System, LOINC Axis 4; Testing; Theoretical model; Time; Unspecified Mental Disorder; Work; design; designing; high risk; interventional strategy; member; men; men`s; mental illness; prevent; preventing; programs; psychological disorder; religious

Project start date: 1985-02-01

Project end date: 2012-02-29

Budget start date: 1-MAR-2010

Budget end date: 28-FEB-2011

5R01HD053314-23 (2010): $568686


Sponsored Links Excellgen http://Excellgen.com

Recombinant Lentivirus & Adenovirus
High Yield and High Titer up to 1010 (lentivirus) and 1013 (adenovirus) for Guaranteed Expression of GOI. $3000, $2500
Transient Protein Expression in CHO and HEK293 Cells
Transient Expression, Truly Functional Protein, 95% purity, 1~20 mg, fast turnaround. $5500, $3950
Baculovirus Protein Expression
Fast turn around, >95% purity functional protein. No outsourcing to China or India. $5500, $3950

Long Term Effects Of Premartial Intervention

Howard J Markm, Professor
Psychologyuniversity Of Denver

Grant 5R01HD053314-22 from Eunice Kennedy Shriver National Institute Of Child Health & Human Development IRG: CLHP

Abstract: The overarching aim of this proposal is to continue a large-scale community based prevention trial of a marriage education program (PREP, Prevention and Relationship Enhancement Program) delivered in Religious Organizations (ROs) to premarital couples. This program is designed to lower risk factors and raise protective factors for marital distress and associated mental health problems. Given the strong links between marital distress and mental disorders, efforts to prevent mental disorder due to marital conflict has substantial advantages over later and more costly treatment. Targeting ROs as a delivery system is important as ROs already serve as a less stigmatizing resource for prevention and counseling for many people who will not seek services from a mental health professional. This work assesses the value of preventing (vs. treating) mental disorders through maximizing functioning during a critical adulthood transition (i.e., entering marriage). This work proposes to follow an existing sample of 217 couples into a high risk period for distress and divorce (years 5-10 of marriage) in order to assess the long-term/durable preventative effects of premarital intervention on marital distress and symptoms of mental disorders. We will also test and refine our theoretical model of how targeted risk (e.g., negative interactions) and protective factors (e.g., confidence in handling conflicts) are linked over time to marital distress and mental disorder. Benefits include enhancing existing "natural" interventions in the community (premarital counseling) and of reaching a population who might not seek services through the traditional mental health system (e.g., men, minority group members)

Keywords: communication behavior, dyadic interaction, education, marriage counseling aggression, conflict, depression, divorce /separation, marriage /marital status, mental disorder, religious group, spouse behavioral /social science research tag, human subject

Project start date: 1985-02-01

Project end date: 2012-02-29