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THE ROLE OF B-CAROTENE IN MATERNAL-FETAL NUTRITION

Loredana Quadro, Phd
Rutgers The St Univ Of Nj New Brunswick, Asb Iii - Cook Campus, New Brunswick, Nj 08901

Grant 5R01HD057493-03 from Eunice Kennedy Shriver National Institute Of Child Health & Human Development

Keywords: 0-11 years old; 3, 7-Dimethyl-9-(2, 6, 6-trimethyl-1-cyclohexen-1-yl)-2, 4, 6, 8-nonatetraen-1-ol; 3, 7-dimethyl-9-(2, 6, 6-trimethyl-1-cyclohexen-1-yl)-2, 4, 6, 8-nonatetraen-1-ol, (all-E)-Isomer; Address; All-Trans-Retinol; Anti-Infective vitamin; Antixerophthalmic vitamin; Autoregulation; Axerophthol; Axerophtholum; Biological; Biosterol; Birth; Birth Defects; Blood; Blood Circulation; Bloodstream; Body Tissues; CMO I; CMO II; Carotene; Carotenes and Carotenoids; Carotenoids; Causality; Cellular Expansion; Cellular Growth; Child; Child Youth; Children (0-21); Circulation; Cleaved cell; Congenital Abnormality; Congenital Anatomic Abnormality; Congenital Anatomical Abnormality; Congenital Defects; Congenital Deformity; Congenital Malformation; Consumption; DAG lipase; Data; Developed Countries; Developed Nations; Development; Diacylglycerol Lipase; Diet; Diet Supplement; Dietary Practices; Dietary Supplements; Diglyceride Lipase; Dose; Egg Yolk; Embryo; Embryo Development; Embryogenesis; Embryonic; Embryonic Development; Embryonic Tissue; Embryonic Tissue, Placenta; Enzymes; Etiology; Fetal Death; Fetus; Genetic Alteration; Genetic Change; Genetic defect; Gestation; Growth and Development; Growth and Development function; Hand; Health; Heparin-Clearing Factor; Homeostasis; Human; Human, Child; Human, General; Incidence; Industrialized Countries; Industrialized Nations; Intake; Intermediary Metabolism; Knowledge; LPL; Lard-Factor; Lipemia-Clearing Factor; Lipoprotein LDL Receptors; Literature; Low Birth Weight Infant; Low Density Lipoprotein Receptor; METBL; Mammals, Mice; Man (Taxonomy); Man, Modern; Maternal Mortality; Metabolic Processes; Metabolism; Metabolism, Lipids/Lipoproteins/Membrane Constituents; Mice; Micronutrients; Molecular Genetic Abnormality; Mothers; Mouse Strains; Murine; Mus; Mutation; Nutrient; Nutritional Supplement; Nutritional status; Oleovitamin A; Ophthalamin; Organ; Oxygenases; Parturition; Physiological Homeostasis; Placenta; Placenta-Tissue, Cells; Placentoma, Normal; Placentome; Plants; Plants, General; Population; Post-Heparin Lipase; Postheparin Lipase; Postheparin Lipoprotein Lipase; Pregnancy; Process; Provitamin A Carotenoid; Public Health; Receptors, LDL; Reticuloendothelial System, Blood; Retinoic Acid Agent; Retinoic Acid and Derivatives; Retinoid Binding Proteins; Retinoids; Retinol Binding Proteins; Retinol-Binding Protein 1; Role; SR-B proteins; Source; Staging; Tissues; Triacylglycero-protein acylhydrolase; United States; VLBW (human); Vitamin A; Vitamin A Alcohol; Vitamin A Deficiency; Vitamin A USP; Vitamins; Yolk Sac; catalyst; cell growth; children; clearing factor lipase; cleaved; corticosterone methyl oxidase I; corticosterone methyl oxidase II; disease causation; disease etiology; disease/disorder etiology; disorder etiology; embryo tissue; fat metabolism; fetal; fetal nutrition; fetus death; fetus nutrition; genome mutation; insight; lipid metabolism; lipoprotein lipase; low birth weight infant human; low birthweight; malformation; mouse development; pi bond; public health medicine (field); retinol; scavenger receptors, class B; social role; triacylglycerol protein acylhydrolase; uptake; vitelline sac; youngster

Project start date: 2008-02-15

Project end date: 2013-01-31

Budget start date: 1-FEB-2010

Budget end date: 31-JAN-2011

PFA/PA: PA-07-070

5R01HD057493-03 (2010): $319512


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The Role Of B-carotene In Maternal-fetal Nutrition

Loredana Quadro
Food Sciencerutgers The St Univ Of Nj New Brunswick

Grant 5R01HD057493-02 from Eunice Kennedy Shriver National Institute Of Child Health & Human Development IRG: INMP

Project start date: 2008-02-15

Project end date: 2013-01-31



Grants awarded to Loredana Quadro

THE ROLE OF B-CAROTENE IN MATERNAL-FETAL NUTRITION

Loredana Quadro, Phd
Rutgers The St Univ Of Nj New Brunswick, Asb Iii - Cook Campus, New Brunswick, Nj 08901

Grant 3R01HD057493-02S1 from Eunice Kennedy Shriver National Institute Of Child Health & Human Development

Abstract: The nutritional status of the mother is a key factor for the development and growth of the fetus. Vitamin A is an essential nutrient for the developing embryo, which depends on maternal circulating vitamin A (or retinoids) to fulfill its needs. Indeed, embryonic vitamin A-deficiency as well as -excess result in fetal malformations, representing a serious problem in both the developing world and the industrialized countries. Humans rely mainly on provitamin A carotenoids as a source of vitamin A. ¿-carotene is the best-characterized vitamin A precursor and the most abundant supply of this nutrient in the human diet. Two enzymes, ¿-carotene-15, 15´-oxygenase (CMO I) and ¿-carotene-9, 10´-oxygenase (CMO II), have been identified as catalysts for the cleavage of specific double bonds of ¿-carotene, resulting in the formation of vitamin A. At present, the role of ¿-carotene in maternal-fetal nutrition is not well understood. Evidences from the literature suggest that this carotenoid may be an important, localized source of vitamin A for the developing tissues. We plan to address this crucial biological question by using mice lacking both CMO I and retinol-binding protein (RBP), the sole specific carrier for retinol in the circulation. We will also use mice lacking low density lipoprotein receptor (LDLr) or class B scavenger receptor (SR-BI) or lipoprotein lipase (LPL) to investigate the role of these key players of the lipid metabolism in facilitating the maternal-fetal transfer of ¿-carotene. Three specific aims are proposed (1) To identify factors that modulate the expression of CMO I and CMO II during mouse development. (2) To determine whether ¿-carotene is an alternative source of vitamin A for embryonic synthesis of retinoids. (3) To investigate the mechanisms of uptake and processing of ¿-carotene by the maternal-fetal barrier (i.e., placenta and yolk sac). These studies will ultimately expand our knowledge of maternal-fetal nutrition and dietary contribution to embryonic development, thus providing new insight into appropriate dietary practices during pregnancy. These studies have a significant impact on human health, since they aim at reducing the incidence of fetal malformations associated with an abnormal maternal intake of micronutrients, such as vitamins

Keywords: 0-11 years old; 3, 7-Dimethyl-9-(2, 6, 6-trimethyl-1-cyclohexen-1-yl)-2, 4, 6, 8-nonatetraen-1-ol; 3, 7-dimethyl-9-(2, 6, 6-trimethyl-1-cyclohexen-1-yl)-2, 4, 6, 8-nonatetraen-1-ol, (all-E)-Isomer; Address; All-Trans-Retinol; Anti-Infective vitamin; Antixerophthalmic vitamin; Autoregulation; Axerophthol; Axerophtholum; Biological; Biosterol; Birth; Birth Defects; Blood; Blood Circulation; Bloodstream; Body Tissues; CMO I; CMO II; Carotene; Carotenes and Carotenoids; Carotenoids; Causality; Cellular Expansion; Cellular Growth; Child; Child Youth; Children (0-21); Circulation; Cleaved cell; Congenital Abnormality; Congenital Anatomic Abnormality; Congenital Anatomical Abnormality; Congenital Defects; Congenital Deformity; Congenital Malformation; Consumption; DAG lipase; Data; Developed Countries; Developed Nations; Development; Diacylglycerol Lipase; Diet; Diet Supplement; Dietary Practices; Dietary Supplements; Diglyceride Lipase; Dose; Egg Yolk; Embryo; Embryo Development; Embryogenesis; Embryonic; Embryonic Development; Embryonic Tissue; Embryonic Tissue, Placenta; Enzymes; Etiology; Fetal Death; Fetus; Genetic Alteration; Genetic Change; Genetic defect; Gestation; Growth and Development; Growth and Development function; Hand; Health; Heparin-Clearing Factor; Homeostasis; Human; Human, Child; Human, General; Incidence; Industrialized Countries; Industrialized Nations; Intake; Intermediary Metabolism; Knowledge; LPL; Lard-Factor; Lipemia-Clearing Factor; Lipoprotein LDL Receptors; Literature; Low Birth Weight Infant; Low Density Lipoprotein Receptor; METBL; Mammals, Mice; Man (Taxonomy); Man, Modern; Maternal Mortality; Metabolic Processes; Metabolism; Metabolism, Lipids/Lipoproteins/Membrane Constituents; Mice; Micronutrients; Molecular Genetic Abnormality; Mothers; Mouse Strains; Murine; Mus; Mutation; Nutrient; Nutritional Supplement; Nutritional status; Oleovitamin A; Ophthalamin; Organ; Oxygenases; Parturition; Physiological Homeostasis; Placenta; Placenta-Tissue, Cells; Placentoma, Normal; Placentome; Plants; Plants, General; Population; Post-Heparin Lipase; Postheparin Lipase; Postheparin Lipoprotein Lipase; Pregnancy; Process; Provitamin A Carotenoid; Public Health; Receptors, LDL; Reticuloendothelial System, Blood; Retinoic Acid Agent; Retinoic Acid and Derivatives; Retinoid Binding Proteins; Retinoids; Retinol Binding Proteins; Retinol-Binding Protein 1; Role; SR-B proteins; Source; Staging; Tissues; Triacylglycero-protein acylhydrolase; United States; VLBW (human); Vitamin A; Vitamin A Alcohol; Vitamin A Deficiency; Vitamin A USP; Vitamins; Yolk Sac; catalyst; cell growth; children; clearing factor lipase; cleaved; corticosterone methyl oxidase I; corticosterone methyl oxidase II; disease causation; disease etiology; disease/disorder etiology; disorder etiology; embryo tissue; fat metabolism; fetal; fetal nutrition; fetus death; fetus nutrition; genome mutation; insight; lipid metabolism; lipoprotein lipase; low birth weight infant human; low birthweight; malformation; mouse development; pi bond; public health medicine (field); retinol; scavenger receptors, class B; social role; triacylglycerol protein acylhydrolase; uptake; vitelline sac; youngster

Project start date: 2009-09-30

Project end date: 2010-09-29

Budget start date: 30-SEP-2009

Budget end date: 29-SEP-2010

PFA/PA: PA-07-070

3R01HD057493-02S1 (2009): $33990