Protein Production
293FT, 293E, CHO

Truly Functional Protein
95% Purity
1-10 mg in 2 weeks

GeneExpressoMax™
293Expresso™

Transfection Reagents
* 90% Efficiency
* 95% Viability
* No sera interference
* Simple protocol
* High-throughput
* Only $98/ml

Baculovirus
Functional Protein
95% Purity
Fast turnaround
1-10 mg from Sf9 cells

Adenovirus, AAV
& Lentivirus

ORF or shRNA
* High Titer
* Cre, FLP, ΦC31
* Protein Kinases
* Transcription Factors
* Luciferases, GFP, RFP
* Protein Production
* Stable Cell Line


Excellgen

Timothy M Dore
University Of Georgia (uga)

Project start date: 2010-02-15

Project end date: 2015-01-31


Sponsored Links Excellgen http://Excellgen.com

Recombinant Lentivirus & Adenovirus
High Yield and High Titer up to 1010 (lentivirus) and 1013 (adenovirus) for Guaranteed Expression of GOI. $3000, $2500
Transient Protein Expression in CHO and HEK293 Cells
Transient Expression, Truly Functional Protein, 95% purity, 1~20 mg, fast turnaround. $5500, $3950
Baculovirus Protein Expression
Fast turn around, >95% purity functional protein. No outsourcing to China or India. $5500, $3950


Grants awarded to Timothy M Dore

LIGHT DRIVEN TOOLS FOR DEVELOPMENTAL NEUROPHYSIOLOGY

Timothy M Dore, Associate Professor
University Of Georgia (uga), Office Of Sponsored Programs, Athens, Ga 30602-7411

Grant 1R01NS070159-01 from National Institute Of Neurological Disorders And Stroke

Abstract: To understand how information propagates in a vertebrate brain requires the ability to accurately control neural activity at the cellular scale in an intact brain. This proposal seeks to create a set of new light-driven technologies for studying the mechanisms involved in the propagation of coherent neural activity in the brain. Because coherent activity is a hallmark of epileptic seizures, this work has direct relevance to understanding epilepsy. Additionally, because substances of abuse modulate specific neural populations, our investigation of how modulating the excitability of particular neuronal populations may shed light on the mechanisms of action of abused substances and new methods of treatment. Specifically, we will synthesize small organic molecules that are capable of delivering neurotransmitters with pinpoint accuracy and timing to specific regions of the brain. These compounds will be used in zebrafish to control neural activity. We will also create modulators of gene expression that can be activated with light. One strategy will silence a gene of interest, whereas the other will be able to activate one. These light driven systems of gene expression will be used in zebrafish to study the development of the central nervous system. The research in this proposal will provide a set of tools that will aid work to understand the mechanisms involved in the propagation of coherent neural activity in the brain and the modulation of particular neuronal populations. Coherent neural activity is a hallmark of epileptic seizures and substances of abuse modulate specific groups of neurons, so this work is relevant to understanding epilepsy and developing methods of treatment for drug abuse. It will also provide a set of tools to explore the role of certain genes in vertebrate development, which will impact work to understand diseases caused by developmental genetic defects

Keywords: No Project Terms available

Relevance: 7. The research in this proposal will provide a set of tools that will aid work to understand the mechanisms involved in the propagation of coherent neural activity in the brain and the modulation of particular neuronal populations. Coherent neural activity is a hallmark of epileptic seizures and substances of abuse modulate specific groups of neurons, so this work is relevant to understanding epilepsy and developing methods of treatment for drug abuse. It will also provide a set of tools to explore the role of certain genes in vertebrate development, which will impact work to understand diseases caused by developmental genetic defects

Project start date: 2010-02-15

Project end date: 2015-01-31

Budget start date: 15-FEB-2010

Budget end date: 31-JAN-2011

PFA/PA: PA-07-070

1R01NS070159-01 (2010): $303625