Brian L Hjelle
University Of New Mexico
Project start date: 1998-09-30
Project end date: 2013-08-31
Sponsored Links Excellgen http://Excellgen.com
BIOLOGY OF INFECTIOUS DISEASE AND INFLAMMATION
Brian L Hjelle
University Of New Mexico, Main Campus Preaward, Albuquerque, Nm 87131
Grant 5T32AI007538-13 from National Institute Of Allergy And Infectious Diseases
Abstract: This proposal is a request for continued support of an expanded training grant (TG) in Infectious Disease and Inflammation (IDIP) at the University of New Mexico. In the 10 years since its inception, this training program provided pre- and postdoctoral trainees opportunities to engage in cutting edge, interdisciplinary and technology-centered infectious disease (ID) research and learn from a wide variety of scientists. An underlying theme has been the promotion of interactions between researchers who are using different experimental approaches to study infectious disease, host responses and biothreats. Crossing departmental and institutional lines, the TG now draws upon the expertise of thirty-two funded investigators. The training faculty have appointments in four School of Medicine departments that include Internal Medicine (8), Cell Biology and Physiology (1), Pathology (9) and Molecular Genetics and Microbiology (7); the College of Pharmacy (1); the UNM department of Chemical and Nuclear Engineering (1); and Lovelace Respiratory Research Institute (5). Faculty trainers fall into four areas of interest 1) Translational Studies in ID and Immunity -bringing basic findings into clinical research, then to practice; 2) Cell Biology of Infection the study of ID at the intercellular level; 3) Pathogenesis of Emerging ID -pathogenic mechanisms of disease causation and 4) Bridging Technologies - the incorporation of cutting edge technologies to the study of ID. Both predoctoral and postdoctoral trainees are expected to complete IDIP-specific courses in addition to their regular (predoctoral) course work in the Biomedical Sciences Graduate Program including specific journal clubs and works in progress sessions. Despite the youth of our TG, we have enjoyed substantial success at recruiting members of under- represented minorities (URM) to the program, and our graduates, including members of minority groups, have already begun to appear among the ranks of the faculty of institutions such as the University of Kentucky or the Scripps Research Institute. We believe our TG has achieved great momentum and is poised to realize even greater impact with its far larger cadre of trainers and greater access to high-quality minority and non-minority students. Support for our program will yield substantial rewards, expanding the ranks of highly interdisciplinary young investigators
Keywords: Biology; Communicable Diseases; INFLM; Infectious Disease Pathway; Infectious Diseases; Infectious Diseases and Manifestations; Infectious Disorder; Inflammation
Project start date: 1998-09-30
Project end date: 2013-08-31
Budget start date: 1-SEP-2010
Budget end date: 31-AUG-2011
PFA/PA: PA-06-468
5T32AI007538-13 (2010): $180785
5T32AI007538-12 (2009): $246195
Biology Of Infectious Diseases & Inflammation
Brian L Hjelle, Professor
University Of New Mexico Albuquerque Health Sciences Ctr, Financial Srvs Div. Albuquerque, Nm 87131
Grant 5T32AI007538-10 from National Institute Of Allergy And Infectious Diseases IRG: MID
Abstract: This proposal is a request for competitive renewal of a training grant to fund four predoctoral and two postdoctoral trainees in our Infectious Disease and Inflammation Program (IDIP). IDIP is comprised of a group of 19 funded investigators, all listed as trainees on this grant, our trainees, junior faculty (not listed a trainers) and administrative and technical staff who support research in infectious disease and inflammation. The areas of expertise of the faculty can be broadly grouped into four areas of interest Pathogenesis of Reemerging Bacterial Infections (3), Biodefense and Emerging Viral Infections (5), Cancer as a Communicable Viral Disease (3) and Basic Inflammatory and Immunologic Mechanisms (8). The training faculty include 17 who have appointments in four University of New Mexico (UNM) School of Medicine (SOM) departments that include Internal Medicine (4), Molecular Genetics and Microbiology (6), Pathology (6), and Cell biology and Physiology (1)] or at Lovelace Respiratory Research Institute (2). The goals of the training program are to 1) enrich interdisciplinary research in infectious diseases and inflammation by providing venues for research presentations, journal clubs, seminars and course development; 2) provide a rich, cooperative educational research environment with opportunities for both pre- and postdoctoral trainees to learn from a wide variety of scientists, their technicians and other trainees; and 3) to provide professional guidance, including instruction and experiential training in scientific ethics, teaching, and opportunities in diverse scientific careers. The plan is to recruit fellowship trainees from among those predoctoral students who have already matriculated in UNM SOM s interdisciplinary Biomedical Sciences Graduate Program (BSGP) who choose to work in the laboratories of IDIP faculty member. The postdoctoral candidates will be selected from qualified postdoctoral trainees recruited to DIP faculty laboratories. The selected trainees would be mentored (with two to three years of training grant support for predoctoral students and two years for postdoctoral trainees) by their specific IDIP trainer, and their progress will be regularly reviewed by the IDIP steering committee. All would be expected to take IDIP-specific courses, in addition to those courses that the predoctoraI trainees are required to take as specified by the BSGP. The program is strongly supported by both UNM SOM and the LRRI as evidenced by the SOM or LRRI jointly funding fully all first year students and by joint BSGP and departmental support for administration of the program, including all recruiting costs. Our program includes specific and unusual strengths in that trainees are exposed to didactic training and laboratory experiences that are very broad and that expose them to excellent interdisciplinary core facilities, strong biotechnotogy (corporate) collaborations, international programs (such as an ICIDR/Fogarty program), work with highly pathogenic potential bioweapons, superb structural biology and imaging capabilities, and well-developed bioengineering research partnerships.
Project start date: 1998-09-30
Project end date: 2008-08-31
5T32AI007538-10 (2007): $177176
5T32AI007538-09 (2006): $186566
5T32AI007538-08 (2005): $188780
Grants awarded to Brian L Hjelle
Biology Of Infectious Disease And Inflammation
Brian L Hjelle, Professor
Pathologyuniversity Of New Mexico
main Campus Preaward
albuquerque, Nm 87131
Grant 2T32AI007538-11 from National Institute Of Allergy And Infectious Diseases IRG: MID
Abstract: This proposal is a request for continued support of an expanded training grant (TG) in Infectious Disease and Inflammation (IDIP) at the University of New Mexico. In the 10 years since its inception, this training program provided pre- and postdoctoral trainees opportunities to engage in cutting edge, interdisciplinary and technology-centered infectious disease (ID) research and learn from a wide variety of scientists. An underlying theme has been the promotion of interactions between researchers who are using different experimental approaches to study infectious disease, host responses and biothreats. Crossing departmental and institutional lines, the TG now draws upon the expertise of thirty-two funded investigators. The training faculty have appointments in four School of Medicine departments that include Internal Medicine (8), Cell Biology and Physiology (1), Pathology (9) and Molecular Genetics and Microbiology (7); the College of Pharmacy (1); the UNM department of Chemical and Nuclear Engineering (1); and Lovelace Respiratory Research Institute (5). Faculty trainers fall into four areas of interest 1) Translational Studies in ID and Immunity -bringing basic findings into clinical research, then to practice; 2) Cell Biology of Infection the study of ID at the intercellular level; 3) Pathogenesis of Emerging ID -pathogenic mechanisms of disease causation and 4) Bridging Technologies - the incorporation of cutting edge technologies to the study of ID. Both predoctoral and postdoctoral trainees are expected to complete IDIP-specific courses in addition to their regular (predoctoral) course work in the Biomedical Sciences Graduate Program including specific journal clubs and works in progress sessions. Despite the youth of our TG, we have enjoyed substantial success at recruiting members of under- represented minorities (URM) to the program, and our graduates, including members of minority groups, have already begun to appear among the ranks of the faculty of institutions such as the University of Kentucky or the Scripps Research Institute. We believe our TG has achieved great momentum and is poised to realize even greater impact with its far larger cadre of trainers and greater access to high-quality minority and non-minority students. Support for our program will yield substantial rewards, expanding the ranks of highly interdisciplinary young investigators
Project start date: 1998-09-30
Project end date: 2013-08-31
2T32AI007538-11 (2008): $239304
Brian L Hjelle, Professor And Interim Chair
University Of New Mexico, Main Campus Preaward, Albuquerque, Nm 87131
Grant 5U19AI045452-10_9001 from National Institute Of Allergy And Infectious Diseases
Abstract: The Virology Core involves laboratories both in Chile and Panama. All virological core services required to support projects 1-3 in Chile are fully operational. Personnel trained during the initial ICIDR grant are in place and assays have been validated. They are executed now on a routine basis. A new BSL-3 laboratory has just been completed at Catholic University (PUC) and another at Development University (UDD) will be ready by June 2005. Western strip immunoblot assays (SIAs) for specific IgM and IgG to diagnose Andes virus infection in humans are now run at clinical centers in the nine geographic regions of Chile where HCPS occurs. Antigens are prepared and supplied by PUC. Thus diagnosis of disease for the purpose of enrollment of patients in projects 2 and 3 does not have to wait for answers in laboratories in Santiago. Indeed, these regional centers now perform diagnostic services for other medical centers in each region as a public health service. Real time quantitative RT-PCR is now available to make early diagnoses of Andes virus infection before the onset of clinical illness and to quantitate viral burden in distinct body fluids of patients. The latter method also will be utilized for rodent infections in project 1. HLA typing of patients who suffer HCPS is ongoing in the UDD laboratory. Neutralizing antibody assays have been developed for both Andes (in Chile) and Choclo (in Panama) viruses and the former will be used to guide treatment trials using immune plasma in Chile beginning in year 2. The Choclo test will be important in further investigation of human infection versus clinical disease in Panama. A novel restriction fragment length polymorphism assay has been developed in the Panama core laboratory that clearly distinguishes Choclo and Calabazo infection in rodents. It will be important in furthering ecologic investigations involving these two hantaviruses for project 1
Keywords: ATGN; Andes Virus; Antigens; Assay; Bioassay; Biologic Assays; Biological Assay; Body Fluids; Body Tissues; Chile; Cities; Clinical; Core Facility; DNA Molecular Biology; Detection; Development; Diagnosis; Diagnostic Services; Disease; Disorder; Early Diagnosis; Ecology; Enrollment; Environmental Science; Gamma Globulin, 19S; Gamma Globulin, 7S; Geographic Area; Geographic Locations; Geographic Region; Geographical Location; Grant; Hantavirus; Human; Human Resources; Human, General; IgG; IgM; Immune Plasma; Immunoblotting; Immunoglobulin G; Immunoglobulin M; Immunology; Immunology (Including BRMP); Immunology (NCI Program); Infection; Institutes; Investigation; Investigators; Laboratories; Life; Liquid substance; Mammals, Rodents; Man (Taxonomy); Man, Modern; Manpower; MeSH Descriptors Class 4; Measurement; Medical center; Methods; Molecular Biology; Molecular Biology, Restriction Fragment Length Polymorphisms; Panama; Panamanian; Patients; Public Health Service; Public Health Service (U.S.); RFLP; RT-PCR; RTPCR; Research Personnel; Researchers; Restriction fragment length polymorphism; Reverse Transcriptase Polymerase Chain Reaction; Rodent; Rodentia; Rodentias; Running; Schools, Medical; Science of Virology; Scientist; Serologic; Serological; Services; Testing; Time; Tissues; Training; USPHS; United States Public Health Service; Universities; Viral; Viral Burden; Viral Diseases; Viral Load; Viral Load result; Virology; Virus; Virus Diseases; Viruses, General; base; diagnosis service; disease diagnosis; disease/disorder; early detection; enroll; fluid; geographic site; immunogen; liquid; medical schools; neutralizing antibody; novel; personnel; reverse transcriptase PCR; treatment trial; viral infection; virology; virus infection
Budget start date: 1-MAY-2009
Budget end date: 30-APR-2010
PFA/PA: RFA-AI-04-017
5U19AI045452-10_9001 (2009): $233248
Genetic Tools For Detecting VHF Bioweapons
Brian L Hjelle, Professor
University Of New Mexico Albuquerque Health Sciences Ctr, Financial Srvs Div. Albuquerque, Nm 87131
Grant 5R21AI053400-02 from National Institute Of Allergy And Infectious Diseases IRG: ZAI1
Abstract: The recent deployment of a bioweapon in an attack on the citizens of the United States has moved bioterrorism from the realm of possible to actual threat. This new realization has brought the need for rapid, high-throughput diagnosis, treatment, and vaccination to the forefront. This application concentrates on development of high-throughput detection of live agents of viral hemorrhagic fever, using the highly lethal SNV (Bunyaviridae Hantavirus) as the model organism. Furthermore, the technology we will develop under this funding will also be of considerable value as facilitating the advancement of treatments for hantavirus pulmonary syndrome (HPS), the disease associated with SNV. This application will develop reverse genetics technology for hantaviruses with the initial intent of preparation of cellular reporters that produce a green signal when the cells are exposed to live, replication-competent SNV. This development would have considerable potential for expanding the technology to other agents of viral hemorrhagic fever. We have a specific plan to further develop and commercialize the product after the initial development phase funded by this application and will seek further funding to develop basic information on the replication of hantaviruses at the end of the two-year funding cycle.
Keywords: Hantavirus, biohazard detection, hemorrhagic fever, monitoring device, technology /technique development, transfection /expression vector, bioterrorism /chemical warfare, high throughput technology, tissue /cell culture
Project start date: 2002-09-30
Project end date: 2005-08-31
5R21AI053400-02 (2003): $225000
Cell Entry Inhibitors For Sin Nombre Virus
Brian L Hjelle, Professor
University Of New Mexico Albuquerque Health Sciences Ctr, Financial Srvs Div. Albuquerque, Nm 87131
Grant 5U01AI056618-05 from National Institute Of Allergy And Infectious Diseases IRG: ZAI1
Abstract: Many of the pathogens that have properties that would be desirable to a would-be bioterrorist have been little-studied, in part because they cause a relatively limited toll in morbidity and mortality under natural circumstances. In other cases, such as for the North American hantaviruses, poor and medically underserved minority populations are disproportionately affected, making their toll even less visible. Two minority institutions in New Mexico, the University of New Mexico and New Mexico State University, have nevertheless devoted much attention to research on potential weapons of mass destruction (Sin Nombre virus or SNV, anthrax, tularemia, plague, etc) for years, with funding from at least four federal agencies. The hantavirus research portfolio at UNM, for example, has been funded at least $1 million direct/year essentially since its inception in 1994. The anthrax attacks of 2001 demonstrated the willingness and ability of terrorists to deploy bioweapons, which caused panic and severe economic damage. Countermeasures against WMD must be developed quickly to limit the damage exacted by future attacks. This application represents a concerted attack against SNV, a hantavirus that has killed over 100 Americans, including 26 New Mexicans, since its recognition in 1993. We have assembled an interdisciplinary team that pools its resources and skills in novel ways. We have developed interlocking, highly collaborative and cross-informing projects and cores involving project PIs who are genuine leaders in their fields, including several who have worked together productively in the past. The approaches that we have taken in this application will serve as a model that can be adapted successfully to a variety of pathogens other than hantaviruses. By pioneering new collaborative strategies using the SNV model, we will establish efficient, high-throughput methods that will work well against other agents. The core capabilities we describe herein will include high-throughput screening for antiviral effects of drugs, computer-assisted modeling that will be used to quickly refine and optimize lead compounds, and evaluation of the efficacy of drugs with sophisticated and quantitative animal model testing. The projects include (1) phage display to develop lead compounds that inhibit SNV entry into cells; (2) computational modeling of drugs; (3) development of RNA aptamer inhibitors of replication and/or packaging using SELEX; (4) development of potent human anti-SNV monoclonal antibodies; and (5) development of sophisticated mechanism-of-action assays to speed development of replicative inhibitors of SNV. Animal model, virology and structural analyses cores will each provide multiple projects with critical reagents or perform the molecular modeling services that are needed to improve lead compounds.
Keywords: Sin Nombre virus, antiviral agent, biotherapeutic agent, drug discovery /isolation, integrin, virus infection mechanism, virus receptor, virus replication, cooperative study, high throughput technology, molecular dynamics, nonhuman therapy evaluation, ribavirin, virion, virus load, Peromyscus, bioterrorism /chemical warfare, flow cytometry, laboratory mouse, monoclonal antibody
Project start date: 2003-09-15
Project end date: 2008-08-29
5U01AI056618-05 (2007): $246761
5U01AI056618-04 (2006): $248018
5U01AI056618-03 (2005): $247632
5U01AI056618-02 (2004): $240843
1U01AI056618-01 (2003): $116915
PROSPECTIVE NATURAL HISTORY STUDY OF HOUSEHOLD CONTACTS OF PERSONS W/ ACUTE HCPS
Brian L Hjelle, Professor And Interim Chair
University Of New Mexico, Main Campus Preaward, Albuquerque, Nm 87131
Grant 5U19AI045452-10_0003 from National Institute Of Allergy And Infectious Diseases
Abstract: Hantaviruses are worldwide in distribution and cause two severe acute diseases of man, hemorrhagic fever with renal syndrome of the Old World and hantavirus cardiopulmonary syndrome of the New World. Chile is among the countries with the highest per capita burden of HCPS in the world in that in a population of about 14 million, it experiences typically 60 to 80 cases of HCPS due to Andes hantavirus per year, with a casefatality ratio of nearly 40%. Among the reasons that Chile experiences more cases than the US is that Andes virus, unlike the etiologic agents of HCPS in the US, is transmitted both by rodent reservoir hosts but also via interpersonal spread. As a result, in Chile there are manyfold greater numbers of "clustered" cases of HCPS in which a single case within a household is followed by secondary cases involving other persons, a phenomenon that is very rarely seen outside of South America. We have recently discovered that interpersonal spread of Andes virus among members of households greatly disproportionately affects the sexual partners of index cases while relatively sparing children and other household members. We believe that these cases can be prevented, but at present almost nothing is known about how they are occurring. We intend to conduct a prospective study of such secondary cases, those that involve sexual contacts of index cases. The goals are to determine the precise modes and risk factors for secondary spread of Andes virus among sexual partners of index cases, to understand what steps are involved in transmission and disease due to the virus, and ultimately to design new interventions to prevent further such tragic transmissions of this highly lethal infection
Keywords: 0-11 years old; Acute; Acute Disease; Affect; Andes Virus; Antibodies; Antigens, Viral; Area; Argentina; Autopsy; Bladder; Blood; Body Fluids; Body Tissues; Cardiopulmonary; Characteristics; Child; Child Youth; Children (0-21); Chile; Clinical; Counseling; Country; Country of Argentina; Data; Dermal; Development; Diagnostic; Disease; Disease Outbreaks; Disorder; Duct; Duct (organ) structure; Ecology; Environmental Science; Epidemiologic Research; Epidemiologic Studies; Epidemiological Studies; Epidemiology; Epidemiology Research; Exposure to; Family; Fever; Funding; Gamma Globulin, 19S; Gamma Globulin, 7S; Gene Products, RNA; Genital; Genital Organs; Genital system; Genitalia; Gingiva; Gingival; Goals; HFRS; Hantavirus; Hantavirus Infections; Head and Neck, Saliva; Head and Neck, Salivary Glands; Health; Heart; Hemorrhagic Fever with Renal Syndrome; Hemorrhagic Fever, Epidemic; Hemorrhagic Fever, Korean; Hemorrhagic Nephroso-Nephritis; Home; Home environment; Household; Human; Human, Child; Human, General; Hyperthermia; IgG; IgM; Immunoglobulin G; Immunoglobulin M; Infection; Inflammatory; Intervention; Intervention Strategies; Investigation; Kidney; Laboratories; Lead; Learning; Light; Link; Liver; Lung; Mammals, Rodents; Man (Taxonomy); Man, Modern; Method LOINC Axis 6; Methodology; Natural History; Oral; Outbreaks; PROV; Panama; Patients; Pb element; Persons; Photoradiation; Physicians; Population; Populations at Risk; Prospective Studies; Province; Pyrexia; Questionnaires; RNA; RNA Viruses; RNA, Non-Polyadenylated; RNA, Viral; Respiratory System, Lung; Reticuloendothelial System, Blood; Reticuloendothelial System, Spleen; Ribonucleic Acid; Risk; Risk Behaviors; Risk Factors; Risky Behavior; Rodent; Rodentia; Rodentias; Rural; Saliva; Salivary Glands; Sexual Partners; Source; South America; Spleen; Symptoms; Syndrome; Testing; Therapeutic Intervention; Thrombocytopenia; Thrombopenia; Tissues; Transmission; Ureter; Urinary System, Bladder; Urinary System, Kidney; Urinary System, Urine; Urine; Viral Antigens; Viral Burden; Viral Load; Viral Load result; Viral Shedding; Viral Sheddings; Viremia; Virus; Virus Shedding; Virus Sheddings; Viruses, General; Visit; acute disease/disorder; acute disorder; at risk behavior; authority; base; body system, hepatic; children; communicable disease transmission; design; designing; disease transmission; disease/disorder; experience; febrile; febris; genital secretion; heavy metal Pb; heavy metal lead; high risk; indexing; infectious disease transmission; intervention therapy; interventional strategy; man; man`s; member; necropsy; neutralizing antibody; oral tissue; organ system, hepatic; postmortem; prevent; preventing; prospective; pulmonary; renal; sample collection; sex partner; specimen collection; tool; transmission process; urinary; urinary bladder; urogenital system (genital part); viraemia; viral RNA; viral sepsis; virus RNA; virus antigen; virusemia; youngster
Budget start date: 1-MAY-2009
Budget end date: 30-APR-2010
PFA/PA: RFA-AI-04-017
5U19AI045452-10_0003 (2009): $93895
SENTINEL SURVEILLANCE FOR FOUR CORNERS HANTAVIRUS
Brian L Hjelle, Professor
University Of New Mexico Albuquerque Health Sciences Ctr, Financial Srvs Div. Albuquerque, Nm 87131
Grant 5R01AI036336-02 from National Institute Of Allergy And Infectious Diseases IRG: EVR
Abstract: Four Corners hantavirus (FCV) is a newly discovered virus that causes chronic infection of the ubiquitous peridomestic rodent Peromyscus maniculatus, and the cause of the highly lethal hantavirus-associated respiratory distress syndrome (HARDS) in humans. Approximately 50% of HARDS cases in the United States have occurred within the state of New Mexico, and the University of New Mexico has become the referral center for many cases that occur outside of New Mexico. American Indian populations are by far the most heavily affected by the HARDS epidemic. Since early June 1993, when the association between FCV and HARDS was recognized, we have (1) developed a PCR-based diagnostic system for patients with possible FCV infection; (2) cloned and sequenced the great majority of the coding sequence for the structural antigens of the virus; (3) developed highly sensitive and specific serologic diagnosis for human and rodent IgM and IgG anti-FCV antibodies, based upon recombinant DNA from FCV and its relatives; and (4) begun preliminary rodent sero- surveillance with the new homologous antigens. We propose to A. Use the newly developed recombinant antigen-derived serologic diagnostic methods to annually monitor seroprevalence of FCV and its relatives in small mammals at three sentinel sites within New Mexico. Correlate seroprevalence data with ecologic measurements and HARDS incidence. B. Test for correlations between ecological parameters such as habitat type, climatological conditions, biomass, and food source availability, and seroprevalence of FCV in rodents. C. Monitor genetic variation of FCV S segment and M segment by PCR in rodents and HARDS patients, to assess the annual rate of mutation and geographic variation among FCV and its relatives. D. Continue to refine and develop our understanding of the molecular structure and antigenicity of FCV, by cloning the remaining region of S segment and the entirety of L segment, and continuing our efforts to use the new clones to further refine the sensitivity and specificity of FCV serologic testing. We will map the dominant epitopes of FCV structural proteins for IgG and IgM antibodies. In collaboration we will develop monoclonal antibodies for direct detection and quantitation of FCV in the tissues and plasma of infected patients. The quantitative information derived from antigen assays will be examined for its predictive value for response to therapy and outcome.
Keywords: Bunyaviridae, adult respiratory distress syndrome, animal ecology, animal population study, epidemiology, epizootiology, serology /serodiagnosis, antiviral antibody, disease reservoir, environment, epitope mapping, evolution, genetic strain, monoclonal antibody, species difference, virus RNA, virus antigen, virus classification, virus genetics, Peromyscus, field study, human data, human subject, molecular cloning, polymerase chain reaction
Project start date: 1994-08-01
Project end date: 1999-04-30
5R01AI036336-02 (1995): $251691
Sponsored Links Excellgen http://Excellgen.com
1R01AI036336-01 (1994): $185639
5R01AI036336-04 (1997): $256763
5R01AI036336-05 (1998): $266834
ECOLOGY OF HANTAVIRUS ENZOOTICS: IMMUNE INTERVENTIONS
Brian L Hjelle, Professor
Pathologyuniversity Of New Mexico
health Sciences Ctr, Financial Srvs Div.
albuquerque, Nm 87131
Grant 5R01AI041692-05 from National Institute Of Allergy And Infectious Diseases IRG: ZRG5
Abstract: A 1993 outbreak of severe respiratory disease among rural residents of the southwestern US was caused by the emergence of a previously-unknown hantavirus, Sin Nombre virus (SNV). Well before SNV was isolated in culture and could be virologically characterized, large amounts of RNA sequence were available from the SNV genome, and used to characterize it antigenically and to develop diagnostic tests. SNV thus represents an excellent model organism for situations in which rapid development and deployment of empirically-designed DNA vaccines could be used to intervene in an outbreak caused by newly-emerged or poorly- characterized pathogens. Virtually nothing is known of how SNV is carried by its reservoir host, the deer mouse. We wish to investigate the mechanisms of transmission of SNV among deer mice in a field setting and to explore mechanisms of immune responses and protection from SNV challenge, using both deer mice and standard laboratory mice (C57/Bl). We will prepare large open-air outdoor enclosures on a patrolled, remote wildlife refuge in western New Mexico to explore transmission of SNV by directly introducing a defined number of infected and uninfected deer mice, which will be fitted with radiotransmitters. The demographics of input rodents will be carefully controlled to investigate the routes of transmission. Their nesting behavior within the enclosure will be monitored by radiotelemetry and correlated with efficiency of transmission of SNV. Studies to determine whether virus-contaminated nest bedding is associated with transmission will also be conducted. Both deer mice and C57/Bl mice will be used to determine the efficacy and immune reactivity to naked DNA immunization using the GI, G2, and N genes of SNV. Lab mice will be used to determine what portions of the SNV genome elicit strong B-cell and T-cell responses, and deer mice will be used to study protection against SNV challenge. Both experimental infection and field transmission challenges will be used. Our long-term goals are to establish the mechanisms by which deer mouse populations maintain SNV infection in natural settings, to identify genetic immunization strategies that may be used to protect at-risk human populations in an outbreak, and to identify ways to disrupt the transmission of SNV among deer mice, and thus to design prevention, control, and treatment strategies
Keywords: Hantavirus, disease /disorder etiology, disease outbreak, disease reservoir, disease vector, ecology, emerging infectious disease, epizootiology, nonhuman therapy evaluation, vector vaccine B lymphocyte, T lymphocyte, antigen antibody reaction, cellular immunity, disease /disorder prevention /control, drug screening /evaluation, ethology Peromyscus, animal breeding, antibody neutralization test, behavioral /social science research tag, enzyme linked immunosorbent assay, field study, laboratory mouse, telemetry, tissue /cell culture, western blotting
Project start date: 1997-08-01
Project end date: 2003-07-31
5R01AI041692-05 (2001): $268624
5R01AI041692-04 (2000): $260882
5R01AI041692-03 (1999): $293689
5R01AI041692-02 (1998): $274532
1R01AI041692-01 (1997): $252592
Prospective Natural History Study Of Household Contacts Of Persons W/ Acute HCPS
Brian L Hjelle, Professor
University Of New Mexico
health Sciences Ctr, Financial Srvs Div.
albuquerque, Nm 87131
Grant 5U19AI045452-090003 from National Institute Of Allergy And Infectious Diseases IRG: ZAI1
Keywords: Hantavirus, South America, cardiopulmonary disease, communicable disease transmission, disease /disorder etiology, disease /disorder proneness /risk, emerging infectious disease, household, interpersonal relations, virus infection mechanism body fluid, cooperative study, neutralizing antibody, sex partner, virus load behavioral /social science research tag, human subject, patient oriented research, polymerase chain reaction
Brian L Hjelle, Professor
University Of New Mexico
health Sciences Ctr, Financial Srvs Div.
albuquerque, Nm 87131
Grant 5U19AI045452-099001 from National Institute Of Allergy And Infectious Diseases IRG: ZAI1
Keywords: Hantavirus, South America, biomedical facility, cardiopulmonary disease, immunology body fluid, cooperative study, histocompatibility typing, neutralizing antibody, restriction fragment length polymorphism immunologic assay /test, polymerase chain reaction, western blotting
Sponsored Links Excellgen http://Excellgen.com
Prospective Natural History Study Of Household Contacts Of Persons W/ Acute HCPS
Brian L Hjelle, Professor
University Of New Mexico Albuquerque Health Sciences Ctr, Financial Srvs Div. Albuquerque, Nm 87131
Grant 5U19AI045452-080003 from National Institute Of Allergy And Infectious Diseases IRG: ZAI1
Keywords: Hantavirus, South America, cardiopulmonary disease, communicable disease transmission, disease /disorder etiology, disease /disorder proneness /risk, emerging infectious disease, household, interpersonal relations, virus infection mechanism, body fluid, cooperative study, neutralizing antibody, sex partner, virus load, behavioral /social science research tag, human subject, patient oriented research, polymerase chain reaction
Brian L Hjelle, Professor
University Of New Mexico Albuquerque Health Sciences Ctr, Financial Srvs Div. Albuquerque, Nm 87131
Grant 5U19AI045452-089001 from National Institute Of Allergy And Infectious Diseases IRG: ZAI1
Keywords: Hantavirus, South America, biomedical facility, cardiopulmonary disease, immunology, body fluid, cooperative study, histocompatibility typing, neutralizing antibody, restriction fragment length polymorphism, immunologic assay /test, polymerase chain reaction, western blotting
Prospective Natural History Study Of Household Contacts Of Persons W/ Acute HCPS
Brian L Hjelle, Professor
University Of New Mexico Albuquerque Health Sciences Ctr, Financial Srvs Div. Albuquerque, Nm 87131
Grant 5U19AI045452-070003 from National Institute Of Allergy And Infectious Diseases IRG: ZAI1
Keywords: Hantavirus, South America, cardiopulmonary disease, communicable disease transmission, disease /disorder etiology, disease /disorder proneness /risk, emerging infectious disease, household, interpersonal relations, virus infection mechanism, body fluid, cooperative study, neutralizing antibody, sex partner, virus load, behavioral /social science research tag, human subject, patient oriented research, polymerase chain reaction
Brian L Hjelle, Professor
University Of New Mexico Albuquerque Health Sciences Ctr, Financial Srvs Div. Albuquerque, Nm 87131
Grant 5U19AI045452-079001 from National Institute Of Allergy And Infectious Diseases IRG: ZAI1
Keywords: Hantavirus, South America, biomedical facility, cardiopulmonary disease, immunology, body fluid, cooperative study, histocompatibility typing, neutralizing antibody, restriction fragment length polymorphism, immunologic assay /test, polymerase chain reaction, western blotting
Prospective Natural History Study Of Household Contacts Of Persons W/ Acute HCPS
Brian L Hjelle, Professor
University Of New Mexico Albuquerque Health Sciences Ctr, Financial Srvs Div. Albuquerque, Nm 87131
Grant 2U19AI045452-060003 from National Institute Of Allergy And Infectious Diseases IRG: ZAI1
Keywords: Hantavirus, South America, cardiopulmonary disease, communicable disease transmission, disease /disorder etiology, disease /disorder proneness /risk, emerging infectious disease, household, interpersonal relations, virus infection mechanism, body fluid, cooperative study, neutralizing antibody, sex partner, viremia, behavioral /social science research tag, human subject, patient oriented research, polymerase chain reaction
Project start date: 2005-08-01
Project end date: 2010-04-30
Brian L Hjelle, Professor
University Of New Mexico Albuquerque Health Sciences Ctr, Financial Srvs Div. Albuquerque, Nm 87131
Grant 2U19AI045452-069001 from National Institute Of Allergy And Infectious Diseases IRG: ZAI1
Keywords: Hantavirus, South America, biomedical facility, cardiopulmonary disease, immunology, body fluid, cooperative study, histocompatibility typing, neutralizing antibody, restriction fragment length polymorphism, immunologic assay /test, polymerase chain reaction, western blotting
Project start date: 2005-08-01
Project end date: 2010-04-30
ECOLOGY OF HANTAVIRUS ENZOOTICS--IMMUNE INTERVENTIONS
Brian L Hjelle, Professor
Pathologyuniversity Of New Mexico
health Sciences Ctr, Financial Srvs Div.
albuquerque, Nm 87131
Grant 3R01AI041692-01S1 from National Institute Of Allergy And Infectious Diseases IRG: ZRG5
Project start date: 1997-08-01
Project end date: 2001-07-31
3R01AI041692-01S1 (1997): $27557
SENTINEL SURVEILLANCE FOR FOUR CORNERS HANTAVIRUS
Brian L Hjelle, Professor
University Of New Mexico Albuquerque Health Sciences Ctr, Financial Srvs Div. Albuquerque, Nm 87131
Grant 5R01AI036336-03 from National Institute Of Allergy And Infectious Diseases IRG: EVR
Project start date: 1994-08-01
Project end date: 1999-04-30
5R01AI036336-03 (1996): $247075